Eggplant is highly responsive to various tissue culture techniques. Somatic embryogenesis and direct organogenesis are widely studied protocols in this crop, but potential of regeneration varies with genotype, explant and culture media supplemented with different combination and concentration of growth hormones. The genotype is the most important factor affecting somatic embryogenesis and organ...

INTRODUCTION Malnutrition, delayed growth and delayed puberty are commonly seen in children with cystic fibrosis. The aim of this study was to evaluate growth, nutritional status and body proportions in children and adolescents suffering from cystic fibrosis. MATERIAL AND METHODS The evaluation was based on 19 somatic measurements and indices calculated from these measurements. Somatic develo...

1. Elemental composition (carbon : nitrogen : phosphorus, C : N : P) was analysed in eggs and juveniles of two crustaceans, Daphnia magna (Cladocera) and the crayfish Astacus astacus (Decapoda). Stoichiometry was also analysed for the carapace, muscle tissue, hepatopanchreas and gills of Astacus. 2. For both species the C : P ratio was significantly higher in eggs than juveniles, but there was ...

Callus was obtained from leaf explants of Cephaelis ipecacuanha on Murashige and Skoog (MS) basal medium supplemented with 0.5 mg/l Kin, 4.0 mg/l 2,4-D and 3% (w/v) sucrose [Murashige, T., Skoog, F., 1962. Plant Physiol. 15, 437±497]. Plantlet regeneration through somatic embryogenesis was achieved on MS medium containing 2.5 mg/l Kin, 1.0 mg/l 2,4-D and 3% (w/v) sucrose. Maturation and germina...

To transfer only part of genome, X-ray irradiated suspension cell protoplasts of N. Ian gsdorfii were fused with suspension cell protoplasts of N. glauca by polyethylene glycol. Somatic hybrid calli were selected by the growth in the hormone-free medium. Some of somatic hybrid calli from fusion with irradiated protoplasts indicated the loss of small subunit polypeptide of fraction 1 protein whi...

Plant tissue culture is an alternative method of commercial propagation and is being used widely for the commercial propagation of a large number of plant species, including many medicinal plants. Somatic embryogenesis is a process by which asexual or somatic cells are induced to form embryos in culture. Somatic embryogenesis is a multi-step regeneration process starting with formation of pro-e...

Induction of somatic embryogenesis in absence of exogenous auxin can clarify some basic concepts of somatic embryogenesis.  Explants from leaf and petiole of Cucumber (Cucumis sativus) were used in this experiment for somatic embryogenesis using three different modified B5 basal media; boron and 2,4-D but no IAA as control (I), boron and IAA but no 2,4-D (II) and no boron, 2,4-D and IAA (III). ...