Frequency of somaclonal variation in embryogenic calli and plants of Olea europaea L cv.Kroneiki derived from somatic embryogenesis were examined by randomly amplified polymorphic DNA (RAPD) analysis. Radicle and cotyledon (proximal part) of mature zygotic embryo were cultured in OMc medium supplemented with IBA (5 mgL-1) and 2-ip (0.5 mgL-1). Somatic embryogenesis was induced in calli of radic...

Objective: n present study effect of various factors including sucrose, agar, PEG, AgNO3, activated charcoal on somatic embryo induction and its subsequent regeneration was investigated using varieties Dom siah and Nemat. Methods: 330 explant (Mature embryos) of each varieties were cultured, 33/plate (100 x 15 mm), on callus induction Murashige and Skoog (MS) medium (Murashige and Sk...

In vitro propagation of C. morifolium cv. ‘Homa’ and cv. ‘Delkash’ and wild C. coccineum via direct and indirect organogenesis and somatic embryogenesis were investigated. BAP at 0, 1, 2 and 3 mg l-1 or NAA at 0, 0.05, 0.1 and 0.2 mg l-1 concentrations were used to induce direct and indirect organogenesis of shoot tip explants. To study the callu...

Somatic embryos and plants were produced from mature and immature embryo derived callus of wheat. Immature embryo showed a better capacity for somatic embryogenesis and plant regeneration. Presence of 2, 4-D was shown to be essential for induction and maintenance of somatic embryogenesis. Organic nitrogenous additives (L-proline, L-asparagine and casein hydrolysate) marked an effect on somatic ...

Immature cotyledons of open-pollinated fruits from three sour cherry cultivars (Prunus cerasus L.) were excised and cultured on Murashige and Skoog medium supplemented with various combinations of auxin and cytokinin to induce somatic embryogenesis. Somatic embryogenesis occurred principally when using the combinations of 2,4-dichlorophenoxyacetic acid plus kinetin. Using a-naphthaleneacetic ac...

The ability to produce morphologically and developmentally normal embryos and, indeed, whole plants from undifferentiated somatic cells in culture, through the process of somatic embryogenesis, resides uniquely within the plant kingdom. Since the initial description of somatic embryo production from carrot callus cells more than 35 years ago (Steward et al., 1958), this unique developmental pot...