Protein disulphide-isomerase from bovine liver was purified to homogeneity as judged by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis, two-dimensional electrophoresis and N-terminal amino acid analysis. The preparative procedure, a modification of that of Carmichael, Morin & Dixon [(1977) J. Biol. Chem. 252, 7163-7167], is much faster and higher-yielding than previous procedures, a...

OBJECTIVE To review the physiology and cardiovascular effects of the commonly used intravenous electrolytes. DATA SOURCES Abstracts, articles and published reviews of studies reported from 1966 to 2000 and identified through a MEDLINE search on cardiac arrhythmias and electrolytes. SUMMARY OF REVIEW While isotonic saline solutions are used to improve the haemodynamic status in critically il...

Polypeptide chain weights of a-, 8and y-gliadin fractions have been estimated by polyacrylamide gel electrophoresis in the presence of mercaptoethanol and sodium dodecyl sulphate. Values lie in the range 32 000 to 44 OOO. When available data on the amino acid analyses were adjusted and compared, significant similarities emerged. It is suggested that these gliadins contain a minimum of 4 cystine...

Reduced and unreduced lysozyme aggregates formed by formaldehyde cross-linking comprise a set of model compounds for studying the effects of protein conformation on the electrophoretic mobilities of sodium dodecyl sulphate-protein complexes. The reduced aggregates were indistinguisable from normal proteins, but the unreduced aggregates migrated anomalously fast by about 14%. Contrary to expecta...

BACKGROUND Successful therapy of leishmaniasis depends on effective cellular immune response. We evaluated the effectiveness of sodium selenite and zinc sulphate as known immunomodulator materials, in combination with Glucantime® in treatment of cutaneous leishmaniasis lesions resulting from Leishmania major in susceptible animal model. METHODS Thirty three female mice weighing 18-20 g at the...

Intact mitochondria, obtained from Euglena gracilis Klebs var. bacillaris Cori mutant W10BSmL, which lacks plastids, and purified on Percoll density gradients, form adenosine 3'-phosphate 5'-phosphosulphate from sulphate. The optimal conditions include addition of 17 mM-Tricine/KOH, pH 7.6, 18 mM-MgCl2, 250 mM-sucrose, 5.66 mM-sodium ADP (or 0.94 mM-sodium ATP), 1 mM-K2SO4, carrier-free 35SO4(2...

Attempts have been made to produce a subunit influenza vaccine containing haemagglutinin and neuraminidase but free of lipid and nucleic acid. Disintegration of virus with Nonidet P40 followed by selective dialysis was only partially successful, but by disintegration with sodium dodecyl sulphate followed by ether extraction and precipitation with ammonium sulphate a product was obtained in whic...

Mining activities are inevitable for the growing industrialization and urbanization in world. Disposal of mine tailings creates a severe impact to environment ultimately society. Identifying applications as potential secondary raw materials would help mining industries achieving circular economy. Alkali activation their utilization building or backfill sites is one such popular technique. In th...

a new polyvinyl choloride (pvc) membrane sensor for cerium (iii) was prepared based on 4,13-didecyl-1,7,10,16-tetraoxa-4,13-diazacyclooctadecane (kryptofix22dd) as an ionophore. the sensor exhibites a linear concentration range of 1.0×10-6to 1.0×10-1m, with a nernstian slope of 18.0±1.0 mv/decade, and a detection limit of 9.7×10-7m. it has a fast response time (5 s) and can be used for two mont...

Estrus cycle has been used as a base to study gustatory responses in rats under conditions of two-bottle choice test given for one hour daily, and the data pooled respectively for diestrus/metestrus (D/M) and proestrus/estrus (P/E). Glucose (13.5%), sodium saccharin (0.2%), sodium chloride (0.9%), citric acid (0.004%) and quinine sulphate (0.002%) was each paired with water and a particular sol...