RNAi has become an essential tool in C. elegans research. This unit describes procedures for RNAi in C. elegans by microinjecting with dsRNA, feeding with bacteria expressing dsRNA, and soaking in dsRNA solution, as well as high-throughput methods for RNAi-based screens.

The use of Argania spinosa press cake for livestock feed is limited due to the presence of saponins, which give it a very bitter flavor and make it unpalatable to livestock. The present study aims to evaluate whether a detoxification method reduces saponin levels of press cake and how that affects its nutrient contents. The response surface methodology was used in this study involved g...

wild almond (amygdalus scoparia) is one of the most important species that provides a variety of ecological functions in the zagros forest ecosystem. in this study, we investigated the effects of some seed treatments on the survival and growth of amygdalus scoparia after seed planting at different aspects and elevations under natural conditions. the seed pretreatments consisted in soaking them ...

Several nematicides have been used to disinfest rice seeds contaminated with the white tip nematode, Aphelenchoides besseyi. We measured the efficacy of a standard disinfestation treatment that consists of soaking seeds in an aqueous emulsion of fenitrothion or fenthion and subsequently air-drying the seeds. We also measured the efficacy of the same treatments but without addition of nematicide...

We investigate the effect of light soaking and forward electric bias treatment on silicon heterojunction solar cells modules and, in particular, influence thermal occurring during lamination. A substantial performance increase is observed after or soaking, which shown to be potentially partly reset by lamination process. This reproduced annealing with same budget. second again improves performa...

individual daily requirements recommend 25-35 g dietary fiber to maintain overall health and aid digestion. dietary fiber exists in fruits and vegetables, but can also be obtained by adding wheat bran to bread. wheat bran, however, contains high levels of phytic acid that is known to chelate iron, calcium, zinc, and magnesium and form complex phytic acid cations that reduce their bioavailabilit...

Structure determination of ligands bound to their target proteins using X-ray crystallography is an important part in the drug discovery process. However, this is not always easy to achieve, especially in cases where the affinity is poor and the solubility of the ligand in the crystallization condition is low. The aim of this project was to investigate a possible correlation between the solubil...