The role of RNA structure in virtually any biological process has become increasingly evident, especially the past decade. However, classical approaches to solving structure, such as crystallography or cryo-EM, have failed keep up with rapidly evolving field and need for high-throughput solutions. Mutational profiling sequencing using dimethyl sulfate (DMS) MaPseq is a sequencing-based approach...

this paper argues for a broader emphasis on sustainable security and smart power, for examining both opportunities as well as threats to security. sustainable security is a new concept in the security studies. deep attention to all possible threats against human life is necessary for continuation of security in a sustainable and dynamic base. this aim can be achieved in a highest level by mergi...

adenylate kinases (adk) are ubiquitous enzymes that contribute to the homeostasis of adeninenucleotides in living cells. in this study, the cloning of a cdna encoding an adenylate kinase from the filariaonchocerca volvulus has been described. using pcr technique, a 281 bp cdna fragment encoding part ofan adenylate kinase was isolated from an o. volvulus cdna library. use of this fragment as a p...

The Smart Grid, regarded as the next generation power grid, uses two-way flows of electricity and information to create a widely distributed automated energy delivery network. In this article, we survey the literature till 2011 on the enabling technologies for the Smart Grid. We explore three major systems, namely the smart infrastructure system, the smart management system, and the smart prote...

Bacteriophages that are routinely used in cDNA libraries do not require any biological selection for forming plaques. Thus parental non-recombinant phages are always found in variable proportions together with recombinant ones in all cDNA libraries. The presence of non-recombinants in significant proportions dilutes the abundance of rare cDNA species and makes library screening difficult. If th...

Some barley mutants can synthesize neither anthocyanins nor proanthocyanidins in the seed coat, which is related to several genes in locus Ant13, but the exact model of action remains unknown. We used the cDNA microarray technology with barley transcription-deficient mutant (ant13-152) that does not synthesize proanthocyanidins as the tester, and its wild type genotype (Triumph) as the driver, ...

We have developed a cDNA library screening method which allows the simultaneous screening of >10 ( 12 ) double-stranded plasmid cDNA molecules with minimal a priori sequence knowledge. A biotinylated, gene-specific oligonucleotide probe along with abutting 'blocking' oligos is hybridized to the plasmid cDNA library and the target plasmid retrieved with paramagnetic streptavidin beads and transf...