multidrug resistance (mdr) is a complex phenomenon in which many different genes regulating drug transport, cellular repair, detoxification and drug metabolism are involved. nevertheless, in most drug resistant cell lines and cancer patients up-regulation of abc-transporter genes such as mdr associated protein (mrp1) gene could be at the basis of the drug resistance phenotype. we aimed to decre...

background: our preliminary data on the protein expression of sort1 in ovarian carcinoma tissues showed that sortilin was overexpressed in ovarian carcinoma patients and cell lines, while non-malignant ovaries expressed comparably lower amount of this protein. in spite of diverse ligands and also different putative functions of sortilin (ntr3), the function of overexpressed sortilin in ovarian ...

Lipid nanoparticles (LNPs) containing short-interfering RNA (LNP-siRNA systems) are a promising approach for silencing disease-causing genes in hepatocytes following intravenous administration. LNP-siRNA systems generated by rapid mixing of lipids ethanol with siRNA aqueous buffer (pH 4.0) where the ionizable lipid is positively charged, followed dialysis to remove and raise pH 7.4. Ionizable c...

background: bone marrow stromal cells (bmsc) have been successfully employed for movement deficit recovery in spinal cord injury (sci) rat models. one of the unsettled problems in cell transplantation is the relative high proportion of cell death, specifically after neural differentiation. according to our previous studies, p75 receptor, known as the death receptor, is only expressed in bmsc in...

multiple gene silencing is being required to target and tangle metabolic pathways in eukaryotes and researchers have to develop a subtle method for construction of rna interference (rnai) cassettes. although, several vectors have been developed due to different screening and cloning strategies but still some potential limitations remain to be dissolved. here, we worked out a simple cloning stra...