Shoot tip and nodal segment explants of Holarrhena antidysenterica when cultured on MS medium containing BAP (1.0-3.0 mg/l) with NAA (0.2-1.0 mg/l) and BAP (1.0-3.0 mg/l) with Kn. (0.2-1.0 mg/l) produced multiple shoots. Maximum multiple shoots was found in MS medium supplemented with BAP (2.0 mg/l) and NAA (0.5 mg/l). Subculture on the same medium resulted in rapid shoot multiplication at an a...

A protocol for micropropagation of Boswellia ovalifoliolata Bal & Henry (Burseraceae) was developed using cotyledonary nodal explant on Murashige and Skoog modified medium (MS). A comparative study of micropropagation with 6-benzyladenine, kinetin and thidiazuron along with 1-naphthalene acetic acid (0.054 microM) was conducted. The highest shoot multiplication (7.1 +/- 0.2 shoots per node) was...

An efficient in vitro propagation system for an elite clone EC-353508 of Artemisia annua L., an important antimalarial drug plant has been developed. The best organogenic response, including adventitious multiple shoots and their elongation, was obtained when nodal explants were cultured on MS medium supplemented with 10μM 2-isopentenyl adenine (2iP). An average of 17.7±0.88 shoots with an aver...

Aseptic cultures of Maranta leuconeura were established from shoot tip explants. They were initiated on Murashige and Skoog (MS)-basal medium supplemented with benzyladenine (BA, 5 mg/l) and agar (6 mg/l) (starting medium). Explants were transferred at 6 week intervals until the onset of proliferation (ca. 6 months). Thereafter, four subcultures were made in liquid multiplication medium contain...

Cecropia glaziovii is a tree with used in Brazilian popular medicine. Methods allowing the clonal propagation of this species are of great interest for superior genotype multiplication and perpetuation. For this reason, we examined the effect of different culture media and different types of explants on adventitious shoot regeneration from callus and buds of C. glaziovii. Leaves, petioles and s...

Withania somnifera (L.) Dunal is an important ayurvedic medicinal plant known mainly for its rejuvenating properties. In vitro micropropagation or multiplication of Withania somnifera through apical shoot proliferation by using different concentrations of growth regulators in short period. Shoots were induced from the apical meristem of Withania somnifera on Murashige and Skoog (MS) basal media...

An efficient in vitro method for multiple shoot bud induction and regeneration has been developed in Artemisia annua L. using leaf and stem explants in various concentrations and combinations of plant growth regulators to evaluate the frequency of regeneration. The sources of explants as well as plant growth regulators in the medium were found to influence the multiple shoot induction. The resu...

Medicinal plants are one of the important sources of natural products, which include essential oils, fragrances, pigments, feedstock, and pharmaceuticals. They are of great interest to the researches in biotechnology field as most of the pharmaceutical industries depend on them Chand et al. [1]. Plumbago zeylanica is an important medicinal plant which belongs to the Plumbaginaceae family. It is...

Micropropagation of hemp ( Cannabis sativa ) is constrained by problems with hyperhydricity and culture decline microshoots. These can be reduced increasing agar nutrients in the media during micropropagation stages 1 2, respectfully. Performance microshoots ‘Abacus’ ‘Wife’ cultured Driver Kuniyuki Walnut medium (DKW) for 15 weeks (6 stage + 9 2), subculturing every 3 both or Murashige Skoog vi...

The main purpose of this article is to review role several organic growth additives such Apple juice, coconut water (CW), maize extract, banana homogenate (BH), peptone and protocorms etc which stimulate the multiplication rate various orchid species in vitro multiplication. These supplements help increase number shoots, root leaf culture medium. In many tissue culture, supplements, are most es...