sugar beetis an essentially self-sterile plant for which the vegetative propagation is used to obtain identical sister plants. sugar beet micropropagation is initiated by meristematic cuttings. sugar beet meristematic flower stalk explants were established in sterile cultures and used for shoot propagation. two pgob medium with different hormonal combinations: 1) ba/naa/iba at 1, 0.1 and 0.1 an...

Background and objectives: Haplophyllum canaliculatum is an endemic and endangered Iranian plant from Rutaceae family. The object of this work was to study the volatile production in established shoot and callus cultures of Haplophyllum canaliculatum as well as isolation, identification and sequencing of 18S rRNA gene from callus culture. Methods: Shoot and callus cultures of H. canaliculatum w...

The vegetative development of the maize shoot can be divided into juvenile and adult phases based on the types of leaves produced at different times in shoot development. Models for the regulation of phase change make explicit predictions about when the identity of these types of leaves is determined. To test these models, we examined the timing of leaf type determination in maize. Clones induc...

Pollen extracts of date palm were used in tissue culture medium for banana as growth substances in comparison with growth regulators. Pollen extracts contain auxin (IBA) and tryptophan (auxin precursor). Water pollen extract (WPE) contains higher concentration of indoles compared with ethanol pollen extract (EPE). Most of treated plants showed highly growth characteristics such as shoot number,...

Calotropis procera, a plant of family Asclepiadaceae is of immense importance as multipurpose plant with medicinal as well as traditional uses. Latex of this plant has been used to cure disease like leprosy, syphilis, inflammation, cutaneous infections, and malarial fever and in many more. In vitro culture of this pharmacologically plant is required otherwise it leads the segregated loss of des...

With the aim of micropropagation of Phyllanthus amarus, an important medicinal herb, shoot tips were cultured in Murashige and Skoog's medium supplemented with kinetin/ BAP singly or in combination with IAA. Growth regulators at lower range (0.1-1.0 mg L(-1)) stimulated direct regeneration of shoots. Kinetin was superior to BAP and kinetin-IAA combination was more suitable than kinetin alone. A...

Shoot tip and nodal segment explants of Holarrhena antidysenterica when cultured on MS medium containing BAP (1.0-3.0 mg/l) with NAA (0.2-1.0 mg/l) and BAP (1.0-3.0 mg/l) with Kn. (0.2-1.0 mg/l) produced multiple shoots. Maximum multiple shoots was found in MS medium supplemented with BAP (2.0 mg/l) and NAA (0.5 mg/l). Subculture on the same medium resulted in rapid shoot multiplication at an a...

In vitro shoot multiplication from nodal explants of Clitoria ternatea was standardized by using MS (Murashige & Skoog) medium supplemented with a cytokinin (BA or Kin). Inclusion of NAA in the culture medium along with BA promoted a higher rate of shoot multiplication. The mean number of shoots per explant after 4 weeks of culture was maximum (5.21) on the MS medium supplemented with 2.0 mg l ...

Origanum acutidens (HAND.-MAZZ.) IETSWAART is a promising ornamental plant that can be widely used in landscape management. It is endemic to Eastern Anatolian region of Turkey. Tissue culture has not been used to micropropagate it. The study reports stem node explants from one-week-old seedlings of the plant for successful micropropagation. The stem nodes were cultured on MS medium containing 0...