Background: Presence of malaria immune factors induced by erythrocytic stages is widely used as an epidemiological approach to diagnose the infection mainly to distinguish the current, recent and past infections. This study was performed to find out the status of malaria, using microscopical and serological (IFA) methods in Bandar-Abbas and Minab, two malarious districts in Hormozgan Province o...

AIM The aim was to evaluate lateral flow assay (LFA) as a field test for investigation of brucellosis outbreak in organized buffalo farm. MATERIALS AND METHODS A total of 153 serum samples were tested to detect the presence of brucella antibodies by LFA and three other serological tests i.e. rose bengal plate test (RBPT), protein G based indirect enzyme-linked immunoassay (iELISA), and compet...

Actinobacillus pleuropneumoniae is a primary bacterial pathogen causing swine respiratory disease and has been described in most major swine-producing regions of the world. Preliminary diagnosis of A. pleuropneumoniae is based on clinical signs and gross pneumonic lesions. Isolating and identifying the causative bacteria confirms the diagnosis. Twelve serotypes of A. pleuropneumoniae and a numb...

Brucellosis has long been recognized with high seroprevalence in Indian dairy herds. Serological surveillance followed regularly to monitor the status of the disease. Four serological methods viz., ELISA, RBPT, STAT and 2-ME tests were used to determine the seroprevalence of brucellosis in an organized dairy farm. Overall, 33.85%, 32.61% and 30.90% animals were diagnosed serologically positive,...

Active and passive protection studies in mice using sheep antisera revealed that the immunological relationship among Pasteurella multocida Type A strains could not be correlated with their serological relationship as determined by a haemagglutination or an agglutination test. Furthermore, strains possessing similar phenol extractable antigens or heat stable antigens did not provide complete cr...

Diagnostic tests for active tuberculosis (TB) based on the detection of antibodies (serological tests) have been commercially available for decades, although no international guidelines have recommended their use. An estimated 1.5 million serological TB tests, mainly enzyme-linked immunosorbent assays, are performed in India alone every year, mostly in the private sector. The cost of serologica...

A collaborative study has been performed to validate two in vitro serological assays, ELISA and ToBI test, as alternatives to the direct challenge procedure for potency testing of tetanus toxoid vaccines for human use (Ph.Eur. monograph Tetanus vaccine (adsorbed) (0452)). In six laboratories, guinea-pigs were immunised with tetanus toxoid vaccines from different manufacturers representing vario...

A total of 1,020 serum and plasma specimens were tested using the Venereal Disease Research Laboratory (VDRL), Rapid Plasma Reagin (RPR) card, Reagin Screen (RST) and Fluorescent Treponemal Antibody-Absorption (FTA-ABS) tests. In 257 normal patients, all screening tests were nonreactive; the FTA-ABS test was reactive for one patient. In 588 patients with treated and untreated syphilis, the RST ...

A new coagglutination kit consisting of a plastic slide with dried antibody-coated staphylococci was evaluated for the grouping of beta-hemolytic streptococci of groups A, B, C, and G. The test was compared with the classical precipitation test, using hot formamide antigen extracts with 224 strains of groups A, B, C, and G streptococci. An agreement of 100% was found between the new coagglutina...