The uptake by murine macrophages of liposomes, exhibiting one of a variety of haptenic groups on their surfaces, was greatly enhanced by the addition of an intact antibody or a lectin specific for the incorporated hapten. The uptake of untreated liposomes was slow and linear over long periods, whereas upon addition of the antibody or lectin, over 30-fold increase in the maximal rate of uptake w...

The rate of uptake and intracellular processing of ligand-directed drug carriers may depend heavily on the endocytic pathway of the target antigen. We examined the role of the target antigen and type of antibodyliposome linkage in determining endocytosis of liposomes by three human I -cell leukemias, Jurkat, CEM, and Molt-4. Liposome-cell binding and internalization over time were studied using...

Liposome-mediated gene transfer is commonly used for in vitro transfection of deoxyribonucleic acid (DNA) into mammalian cells. We and others have recently demonstrated that this can be an effective method for in vivo delivery of plasmid DNA containing the human cystic fibrosis transmembrane conductance regulator (CFTR) gene to mouse models of cystic fibrosis (CF). This suggests that cationic l...

UNLABELLED Cardio-vascular diseases are the main cause of death, emphasizing the need to improve patient treatment and survival. One therapeutic approach is a liposome-based drug carrier system specifically targeting constricted arteries. The recently discovered mechano-sensitive liposomes use hemodynamic shear-stress differences between healthy and constricted blood vessels as trigger for drug...

Liposomes have been used as a vehicle for encapsulating chemicals or toxins in toxicological studies. We investigated the transient effects of empty liposomes on hepatic macrophages by applying a single intravenous injection at a dose of 10 ml/kg body weight in 6-week-old male F344 rats. One day after injection, the numbers of hepatic macrophages reacting to CD163, CD68, Iba-1, MHC class II, Ga...

BACKGROUND Liposomal drug delivery systems, a promising lipid-based nanoparticle technology, have been known to play significant roles in improving the safety and efficacy of an encapsulated drug. METHODS Liposomes, prepared using an optimized proliposome method, were used in the present work to encapsulate piroxicam, a widely prescribed nonsteroidal anti-inflammatory drug. The cytotoxic effe...

After extensive work spanning more than half a century, liposomes have crossed the chasm and emerged as prominent candidate in mainstream drug delivery. Post historic launch of first liposome-based drug, it is last three decades when liposome technology has slowly transformed from research apparatus to versatile carrier system influencing many biomedical areas. Liposomes ability deliver target ...

A single molecule fluorescence assay is presented for studying the mechanism of soluble N-ethyl maleimide sensitive-factor attachment protein receptors (SNAREs)-mediated liposome fusion to supported lipid bilayers. The three neuronal SNAREs syntaxin-1A, synaptobrevin-II (VAMP), and SNAP-25A were expressed separately, and various dye-labeled combinations of the SNAREs were tested for their abili...

omega-3 fatty acids (fas) have been shown to prevent cardiovascular disease. the most commonly used omega-3 fatty acids like eicosapentaenoic acid (epa) and docosahexaenoic acid (dha) are highly vulnerable to oxidation and therefore, have short shelf life. recent advances in nanoliposomes provided a biocompatible system for stabilizing omega-3 fas. several methods could be implemented to prepar...

BACKGROUND Liposome bupivacaine is a prolonged-release bupivacaine formulation indicated for single-dose administration into the surgical site to produce postsurgical analgesia. METHODS An overview of time to onset of analgesia observed with liposome bupivacaine in human studies is provided, as well as a summary of data from pharmacokinetic studies including those that assessed pharmacokineti...