Encapsulation of oligonucleotides in antibody-targeted liposomes (immunoliposomes) which bind to target cells permits intracellular delivery of the oligonucleotides. This approach circumvents problems of extracellular degradation by nucleases and poor membrane permeability which free phosphodiester oligonucleotides are subject to, but leaves unresolved the inefficiency of encapsulation of oligo...

Synthetic CpG oligonucleotides are promising components of immunomodulatory drugs for the treatment and prophylaxis infectious diseases, cancers, allergies. Phosphorothioate modification stabilizes these compounds, contributing to achievement a clinical effect, but at same time changes their properties. We used diffusible fluorescent dye dihydroethidium non-diffusible 6-carboxy-2′,7′dihydrochlo...

A modular approach has been developed for enzymatic ligation of peptides/proteins with oligonucleotides through the design tag phosphoramidites as adaptors, paving way towards streamlined production peptide/protein-oligonucleotide conjugates.

Vimentin intermediate filaments undergo spatial reorganization in cultured smooth muscle cells in response to contractile activation; however, the role of vimentin in the physiological properties of smooth muscle has not been well elucidated. Tracheal smooth muscle strips were loaded with antisense oligonucleotides (ODNs) against vimentin and then cultured for 2 days to allow for protein degrad...

In most human follicular B cell lymphomas the bcl-2 gene is up-regulated as a result of the t(14;18) chromosomal translocation generating a hybrid bcl-2-IgH mRNA. Recently, we have identified in t(14;18)-positive cells a bcl-2-IgH mRNA in the antisense orientation, putatively responsible for the overexpression of bcl-2. Herein we show that this chimeric antisense transcript is an optimal target...

Oligonucleotides are indispensable tools in diagnostics, therapeutic applications and molecular biology. The low base pairing strength of thymine with adenine complicates their use. Ethynylpyridone C -nucleoside analogs thymidine that pair more strongly improved selectivity, sequences containing these show target affinity but routine use is hampered by diminished yields solid-phase syntheses th...

SARS-CoV-2 In their Communication on page 21662, Xinjing Tang et al. report the efficient inhibition of using chimeric antisense oligonucleotides through RNase L activation.