A labile selenium donor compound monoselenophosphate is synthesized from selenide and ATP by selenophosphate synthetase (SPS). In the present study, Sps1 and Sps2 were cloned from a cDNA library prepared from human lung adenocarcinoma cells (NCIH441). The human lung Sps1 has been cloned as an ORF of 1,179 bp, identical in sequence to that of the recently revised human liver Sps1. The in-frame T...

Selenoprotein P (Sepp1) contains most of the selenium in blood plasma, and it is utilized by the kidney, brain, and testis as a selenium source for selenoprotein synthesis. We recently demonstrated that apolipoprotein E receptor-2 (ApoER2) is required for Sepp1 uptake by the testis and that deletion of ApoER2 reduces testis and brain, but not kidney, selenium levels. This study examined the kid...

Not only is fish a good source of protein, but it also provides essential fatty acids such as omega-3, vitamins vitamin D, and minerals selenium. However, consumers are concerned about exposure to mercury through consumption. The degree depends on both the amount type consumed. Additionally, recent studies have shown that Se can prevent poisoning. To better understand risk seafood consumption, ...

Selenium enriched yeast is the most prevalent source of organic selenium for humans and animals. In this study, an effective capillary zone electrophoresis method was established to quantify total in biomass by measuring selenite level post incubated culture media. The detection could be achieved within 7.9 min under condition a mixture buffer 85 mM Na2HPO4 0.5 CTAB (pH 9.37), –5 kV separation ...

Nutrient needs increase during pregnancy and lactation to support fetal growth and milk synthesis, respectively. Physiological adjustments that are made to meet those needs alter the fraction of ingested nutrient retained, or the bioavailability. Using stable isotopes as tracers, we measured calcium, zinc and selenium homeostasis in women during reproduction. The physiological response, and the...

We determined levels of inorganic mercury (I-Hg) and methylmercury in placentas from 119 Swedish women, not selected with respect to high exposure of mercury. Our objective was to relate placental Hg species with maternal and fetal blood concentrations and to evaluate possible associations with selenium. We performed the analyses using automated alkaline solubilization/reduction and cold-vapor ...

Intervention trials with different forms of selenium are under way to assess the effects of selenium supplements on the incidence of cancer and other diseases. Plasma selenium biomarkers respond to selenium administration and might be useful for assessing compliance and safety in these trials. The present study characterized the effects of selenium supplementation on plasma selenium biomarkers ...

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