We investigated the sensitivity of dormant Polish-provenance Fraxinus excelsior L. seeds to extreme desiccation and liquid nitrogen (LN, -196 degrees C). Germination and seedling emergence tests revealed that the critical water content (WC) of the desiccated seeds was 0.04-0.06 g H(2)O g(-1) dry mass, g g(-1) (0.04 g g(-1) in the germination test and 0.06 g g(-1) in the seedling emergence test)...

Adult mesenchymal stem cells (MSCs) have been investigated using preclinical approaches for tissue regeneration. Porcine MSCs (pMSCs) are capable of growing and attaching to plastic with a fibroblast-like morphology and then differentiating into bone, adipose, and cartilage tissues in vitro. This study was conducted to investigate the proliferating abilities, differentiation potentials, and mul...

objectives: freezing and in vitro culture of testicular spermatozoa or tissue are reliable approaches for the management of azoospermic patients and could all for the possibility of multiple ivf-icsi procedures. however, the effect of cryopreservation duration on pregnancy outcome had been not fully examined. to analyze pregnancy outcome in patients underwent icsi procedure in relation to durat...

The present study was designed to test the hypothesis that addition of anticaspase cocktails (inhibiting caspases and thus blocking apoptosis) to the extenders increases the post-thaw viability of equine spermatozoa. The addition of caspase inhibitors failed to improve the acrosome and plasma membrane integrity of spermatozoa, suggesting that in equine sperm cryopreservation protocols, the addi...

OBJECTIVE During IVF, non-transferred embryos are usually selected for cryopreservation on the basis of morphological criteria. This investigation evaluated an application for array comparative genomic hybridization (aCGH) in assessment of surplus embryos prior to cryopreservation. METHODS First-time IVF patients undergoing elective single embryo transfer and having at least one extra non-tra...

In women, around 10% of cancers occur in those younger than 45 years old. Although aggressive chemotherapy/radiotherapy and bone marrow transplantation can cure more than 90% of girls and young women affected by disorders requiring such treatment, the ovaries are very sensitive to cytotoxic drugs, especially to alkylating agents. Therefore, these treatments can result in premature ovarian failu...

Light Source INTRODUCTION This study presents a new device prototype for visualization of physical effects associated with large-scale cryopreservation—the preservation of tissues at very low temperatures. Cryopreservation represents the only method for long-term preservation of biomaterials. While techniques for cryopreservation of single cells and small tissue structures are well established,...

BECAUSE agricultural production is seasonal while food consumptioji is steady, the storage of food in some edible form has always been one of mankind's greatest problems. Practically all natural foodstuffs except ripe seeds undergo decay unless measures are taken to prevent it. Dried fruits, pickles, preserves, cheese, and fermented drinks probably all have their origin in attempts at food pres...

background: an effect of cryopreservation on human sperm is sublethal cryodamage, in which cell viability post-thaw is lost more rapidly at later times than in fresh cells. objective:  this study examined whether the addition of an antioxidant to cryopreservation medium could improve the post-thaw parameters and evaluation of sperm chromatin quality of cryopreserved human spermatozoa from men w...