This work describes the formation of poly(ethylene glycol) (PEG) microgels via a photopolymerized precipitation reaction. Precipitation reactions offer several advantages over traditional microsphere fabrication techniques. Contrary to emulsion, suspension, and dispersion techniques, microgels formed by precipitation are of uniform shape and size, i.e. low polydispersity index, without the use ...

BACKGROUND AND PURPOSE Allergy is an undesired immune response to non-pathogenic agents. However, some opportunistic microorganisms such as fungi can also cause allergy. Among those fungi, hyphae form of Aspergillus strains including A. fumigatus, A. flavus, and A. niger could be mentioned. In this study, we aimed to separate allergic proteins from Aspergillus strains and determine their identi...

Methods to sequentially extract and fractionate wheat flour proteins were evaluated to reliably quantify gliadins, glutenins, and albumins/globulins in single flour samples. Compositions of the resulting protein fractions were analyzed by RP-HPLC combined with SDS-PAGE. Unknown proteins were identified by mass spectrometry or N-terminal sequencing. The best separation and recovery of discrete a...

Animal fleshing, the major proteinaceous solid waste generated from leather industry, was used as substrate for the production of alkaline protease by Bacillus cereus 1173900 in solid-state fermentation (SSF). Maximum protease activity of 12,310 U/g was observed at 60 h in SSF crude extract. The extracted protease enzyme was purified by 53.64-fold through ammonium sulphate precipitation and chr...

In this study chemical method of dissolution-Precipitation was applied to produce amorphous silica nanoparticles from rice straw ash (RSA), the waste material of rice cultivation. The morphology, particle size, structure and area of specific surface of synthesized amorphous silica nanoparticles were evaluated using transmission electron microscopy (TEM), X-ray diffraction analysis (XRD) and BET...

Two-dimensional dendritic growth due to solute precipitation was simulated using a phase-field model reported earlier [Z. Xu and P. Meakin, J. Chem. Phys. 129, 014705 (2008)]. It was shown that diffusion-limited precipitation due to the chemical reaction at the solid-liquid interface has similarities with diffusion-limited aggregation (DLA). The diffusion-limited precipitation is attained by se...

In a case of fatal paraquat poisoning, the distribution of paraquat was determined by a) the column chromatographic method developed by Tompsett, b) the ion-pairing method, c) the deproteinization method both developed by Jarvie et al, and d) the precipitation method developed by the authors. The precipitation method relies on the use of a precipitate of paraquat with Reinecke's salt to extract...

Most methods of estimating the concentration of bilirubin in serum are based on the van den Bergh reaction in which bilirubin couples with diazotized sulphanilic acid to give red " azobilirubin," the reaction being facilitated by the addition of an activator or catalyst such as ethanol. The many modifications of this reaction can be classified into two groups: (a) those in which ethanol is used...

In the present study, sheep crude hydatid cyst fluid antigen (SCHCFA) was prepared by ammonium sulfate precipitation, centrifugation and dialysis. SDS-PAGE of SCHCFA showed seven polypeptides of 72.8 kDa, 66.5 kDa, 60.2 kDa, 41 kDa, 25.1 kDa, 19 kDa and 8 kDa when stained with Coomassie briliant Blue R-250. The initial part of the ascending loop of the first peak (PI) when resolved by gel filtr...

According to the United Nations Sustainable Development Goals (SDG 15.3), frequent sand and dust storms (SDSs) in spring are a long-term challenge prevention control of land degradation on Mongolian plateau. In this study, MODIS remote sensing data used monitor analyse SDS events Plateau. The annual distribution SDSs (March May) from 2000 2021 obtained based storm detection index. overall class...