We report that subunits of human nuclear proteasomes carry a previously unrecognised, constitutive posttranslational modification. Subunits with this modification are not visualised by SDS-PAGE, which is used in almost all denaturing protein gel electrophoresis. In contrast, CTAB-PAGE readily visualises such modified subunits. Thus, under most experimental conditions, with identical samples, SD...

One of the most commonly observed surface structures on the prokaryotic cell envelopes is monomolecular crystalline array of proteinaceous subunits termed Surface Layers or S-layers. Due their self assembly ability and the highly ordered, regular structure down to the nanometer scale, S-layers were demonstrated to possess a great potential for nanobiotechnological applications. Some probiotic b...

Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is commonly used to obtain high resolution separation of complex mixtures of proteins. The method initially denatures the proteins that will undergo electrophoresis. Although covalent structural features of resolved proteins can be determined with SDS-PAGE, functional properties are destroyed, including the presence of non-cov...

odium dodecyl sulfate polyacrylamide gel electrophoresis (SDSPAGE) is the most widely used analytical method to resolve separate components of a protein mixture. It is almost obligatory to assess the purity of a protein through an electrophoretic method. SDS-PAGE simultaneously exploits differences in molecular size to resolve proteins differing by as little as 1% in their electrophoretic mobil...

IB-16385A Abstract Capillary electrophoresis sodium dodecyl sulfate (CE-SDS), is the modern equivalent of the slabgel sizing technique SDS-PAGE. Common uses of SDS-PAGE include monitoring of manufacturing consistency and apparent molecular weight. Although used frequently, SDS-PAGE is a poor technique for quantitative protein purity due to inherent sample preparation artifacts, migration time a...

the genus hyoscyamus belongs to the tribe hyoscyameae miers of solanaceae family. variation in protein bands elaborates the relationship among the collections from various geographical regions. in this study the seed storage protein diversity of 19 accessions of hyoscyamus (h. niger, h. reticulatus and  h. pusillus) from west azerbaijan (iran) was investigated according to the sodium dodecyl su...

ϕEf11, a temperate Siphoviridae bacteriophage, was isolated by induction from a root canal isolate of Enterococcus faecalis. Sequence analysis suggested that the ϕEf11 genome included a contiguous 8 gene module whose function was related to head structure assembly and another module of 10 contiguous genes whose products were responsible for tail structure assembly. SDS-PAGE analysis of virions ...

background and objectives: the linear epitopes of gp46-i, gp46-ii, gp21 and p19 are used in diagnosis of htlv-i/-ii infections. the aims of this study was to obtain high-level expression and purification of recombinant antigen (ra) containing these epitopes. large-scale preparation of such antigen probably worths for diagnostic purpose. materials and methods: the synthetic dna encoding ra was s...