A system was developed for the rapid characterization of microbial pectic enzyme complexes and then tested on Erwinia chrysanthemi and Sclerotium rolfsii. Pectic enzymes in minute samples of crude culture filtrates were resolved by ultrathin-layer polyacrylamide gel isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis and then assayed with an ultrathin pectate-agar...

Mycorrhizal spores were found to be closely associated with bacteria on their spore wall surface known as mycorrhiza associated bacteria (MAB) and its play a major role in the plant growth promotion by production of many known and unknown metabolites, nutrient solubilization and suppression of many soil borne pathogens in rhizosphere of crop plants. The present study was carried out to understa...

BACKGROUND Sclerotium rolfsii Sacc. is a destructive soil-borne plant pathogen that infects over 500 plant species and causes significant yield losses in many economically important plant species. Synthetic fungicides used to combat the menace also pollute the environment and cause health hazards. In order to search environmental friendly alternatives from natural resources, methanolic extracts...

In vitro bio-efficacy of the novel copper (NC101 and NCP102) phosphonate (PN103 PMN104) based fungicides against various soil borne grape vine pathogens was evaluated at ICAR-National Research Centre for Grapes, Pune, Maharashtra, India during February - April 2021. The were screened five bacterial viz. Xanthomonas campestris pv. citri, X. campestris, punicae, viticola oryzae 10 fungal Rhizocto...

This study aims at identifying the major soil borne pathogens of tomato associated with damping-off in nursery areas. To achieve this, nurseries plant pathology farm our area has been investigated. Samples symptoms were collected and cultured under aseptic conditions. Fungi isolated from diseased stem roots on potato dextrose agar (PDA) medium. Growing colonies fungi purified by single spore hy...

Sclerotium rolfsii (Sacc.) is a serious plant pathogenic fungus and lacks perfect (basidial) stage in production. Protoplast fusion technology was employed to reconstruct fusants from this fungus. Two strains designated as A and R were used. Maximum protoplast yields of 3.8x10(5)/g mycelia and 2.8x10(5)/g mycelia were formed in strains A and R respectively. Osmotic stabilizer sucrose 1M gave ma...