In the budding yeast Saccharomyces cerevisiae, cell cycle initiation is prompted during G(1) phase by Cln3/cyclin-dependent protein kinase-mediated transcriptional activation of G(1)-specific genes. A recent screening performed to reveal novel interactors of SCB-binding factor (SBF) and MCB-binding factor (MBF) identified, in addition to the SBF-specific repressor Whi5 and the MBF-specific core...

In yeast, the G1 cyclin Cln3 promotes cell cycle entry by activating the transcription factor SBF. In mammals, there is a parallel system for cell cycle entry in which cyclin dependent kinase (CDK) activates transcription factor E2F/Dp. Here we show that Cln3 regulates SBF by at least two different pathways, one involving the repressive protein Whi5, and the second involving Stb1. The Rpd3 hist...

Superficial bacterial folliculitis (SBF) is more common in the dog than other mammalian species. Until recently, a successful outcome in cases of canine SBF was possible by administering a potentiated amoxicillin, a first generation cephalosporin or a potentiated sulfonamide. Unfortunately, this predictable susceptibility has changed, because methicillin resistant Staphylococcus pseudintermediu...

An important issue in teaching interdisciplinary biologically inspired design is the external representations we use to foster understanding of biological systems. In this study we explore if functional models of biological systems, and in particular Structure-BehaviorFunction (SBF) models, enable humans to better understand complex biological systems. The study compares the use of SBF models i...

Surface modification of titanium was investigated by means of hydrothermal treatment with a maximum pressure of 6.3 MPa (280 degrees C temperature) in CaO solution or water to improve bioactivity and biocompatibility. As a result, calcium titanate was formed on the titanium surface. Moreover, titanium oxide and titanium hydroxide layers on the surface increased as temperature and pressure incre...

The classic biomimetic apatite coating process can be accelerated by first immersing substrates into concentrated simulated body fluid, 5x SBF (SBF1), at 37 degrees C, to form an initial coating of precursor apatite spheres, and subsequently transferring to a second 5x SBF (SBF2) solution which is devoid of crystal growth inhibitors to promote phase transformation of SBF1-derived precursor apat...

Dense, polycrystalline, synthetic hydroxyapatite (HA) was incubated for 36 days in modified simulated body fluid (SBF) with increased HCO3 and reduced Cl ion concentrations (27 and 120 mM, respectively) closer to actual blood plasma than typical SBF. The resulting precipitated apatite layer was characterized by X-ray photoelectron spectroscopy (XPS) and contact angle measurements and found to b...

OBJECTIVE To compare the effects of select leg compression pressures on blood perfusion in the skin overlying bone and the skin distal to the leg compression. DESIGN Blood perfusion of skin overlying the tibia and the foot dorsum was simultaneously measured in 12 healthy subjects using laser Doppler. Each subject's calf was compressed from ankle to knee with an air cast that applied external ...

Abstract Bioglass 45S5 is the most widely used bioactive glass composition in orthopedics to regenerate bone tissue. Although its reactions vitro and vivo are well established, impact of local environment on dissolution behavior dynamic not fully understood. Here, we show that ion concentrations released from into Tris‐buffer solution simulated body fluid (SBF) a cascade system three reactors s...

Autophagy, the process for recycling cytoplasm in the lysosome, depends on membrane trafficking. We previously identified Drosophila Sbf as a Rab21 guanine nucleotide exchange factor (GEF) that acts with Rab21 in endosomal trafficking. Here, we show that Sbf/MTMR13 and Rab21 have conserved functions required for starvation-induced autophagy. Depletion of Sbf/MTMR13 or Rab21 blocked endolysosoma...