The tyrosine kinase (TK) family includes many growth factor receptors, cell cycle regulators, and oncoproteins. Moreover, the receptor TKs HER2/neu and epidermal growth factor receptor are overexpressed in a subgroup of breast tumors and correlate with more aggressive behavior. Thus, TKs are being actively pursued as therapeutic targets. The purpose of this study was to determine the expression...

25 Most functional metagenomic studies have been limited by the poor expression of many genes 26 derived from metagenomic DNA in Escherichia coli, which has been the predominant surrogate 27 host to date. To expand the range of expressed genes, we developed tools for construction and 28 functional screening of metagenomic libraries in Streptomyces lividans. We expanded on 29 previously publishe...

Unique DNA sequences can be determined directly from mouse genomic DNA. A denaturing gel separates by size mixtures of unlabeled DNA fragments from complete restriction and partial chemical cleavages of the entire genome. These lanes of DNA are transferred and UV-crosslinked to nylon membranes. Hybridization with a short 32P-labeled singlestranded probe produces the image of a DNA sequence "lad...

We describe a highly redundant murine genomic library in a new λ phage, λ knockout shuttle (λKOS) that facilitates the very rapid construction of replacement-type gene targeting vectors. The library consists of 94 individually amplified subpools, each containing an average of 40 000 independent genomic clones. The subpools are arrayed into a 96-well format that allows a PCR-based efficient reco...