Antibiotic biosynthesis in the streptomycetes is a complex and highly regulated process. Here, we provide evidence for the contribution of a novel genetic locus to antibiotic production in Streptomyces coelicolor. The overexpression of a gene cluster comprising four protein-encoding genes (abeABCD) and an antisense RNA-encoding gene (α-abeA) stimulated the production of the blue-pigmented metab...

This paper addresses the problem of residential waste collection, as a real life application of a sectoringarc routing problem (SARP). Tactical decisions comprise the partition of the service territory into a number of sectors so that each sector can be covered by a set of vehicle trips. In addition, operational decisions involving the design of the vehicle trips that minimize total routing tim...

Sequencing of the DNA region on the left fringe of the pimaricin gene cluster revealed the presence of a 3.6-kb gene, pimR, whose deduced product (1,198 amino acid residues) was found to have amino acid sequence homology with bacterial regulatory proteins. Database comparisons revealed that PimR represents the archetype of a new class of regulators, combining a Streptomyces antibiotic regulator...

The root of the eukaryote tree of life defines some of the most fundamental relationships among species. It is also critical for defining the last eukaryote common ancestor (LECA), the shared heritage of all extant species. The unikont-bikont root has been the reigning paradigm for eukaryotes for more than 10 years but is becoming increasingly controversial. We developed a carefully vetted data...

of Dissertation Presented to the Graduate School of the University of Florida in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy PERFORMANCE MODELING AND ANALYSIS OF MULTICAST INFRASTRUCTURE FOR HIGH-SPEED CLUSTER AND GRID NETWORKS By Hakki Sarp Oral December 2003 Chair: Alan D. George Major Department: Electrical and Computer Engineering Cluster computing provide...

This guidebook is part of an ongoing series intended to assist public agencies, non-profit organizations, and the private sector with design and implementation of water acquisition programs to improve water supply reliability during drought and under climate change. While this guidebook addresses the use of auctions, others being developed will discuss water supply reliability contracts, region...

Eggs are an important and convenient source of protein for those living in rural regions. In addition, eggs from local hens popular traded a fair price. As result, the primary goal this study was to evaluate egg laying quality parameters Sakini (Gallus gallus domesticus) Nepal order better understand chicken’s reproductive fitness relationship between parameters. For this, total 120 different w...