Benzaldehyde (Bz) is a typical fragrant compound for peach-flavored beverages. In the food and beverage industries there is great demand for a sensitive and easy detection system of Bz in order to ensure product quality control and to avoid contamination. For the noncompetitive detection of Bz, we applied an open-sandwich enzyme-linked immunosorbent assay (OS-ELISA) utilizing an antigen-depende...

Hydatidosis, caused by the larval stage of Echinococcus granulosus, is one of the most important zoonosis with worldwide distribution. As its diagnosis by clinical symptoms and scanning alone is difficult and confusing, we designed the present study to achieve a sensitive and simple diagnostic method for epidemiological studies. Sera (250 samples) were collected from 90 cases of hydatidosis pro...

Trace amounts of cashew nut protein can provoke severe allergic reactions in sensitive patients. Consequently, commercial food processors and regulatory agencies must be vigilant to prevent cashew nut cross-contamination among foods and ensure proper labeling. Toward this end, we have developed a sandwich enzyme-linked immunosorbent (ELISA) to detect the predominant cashew protein fraction (ana...

Murine monoclonal antibodies to target Salmonella typhimurium flagellin and lipopolysaccharide (LPS) were prepared and characterized. For the immunological detection of S. typhimurium, different pairs of monoclonal antibodies (MAbs) were tested in a sandwich enzyme linked immunosorbent assay (ELISA) format. After comparison, a sandwich ELISA and immunochromatographic strip based on LPS MAbs was...

We offer eight well-characterized anti-SAA MAbs for the detection of feline, canine and equine SAA. These MAbs were selected from a pool of over forty MAbs that were raised against human SAA (SAA19, SAA21, SAA11), canine SAA (VSA34, VSA31, VSA38), or synthetic peptides derived from the region 79-104 a.a.r. of canine SAA (VSA2, VSA43). Isotypes and specificities of the antibodies are shown in Ta...

We previously identified novel biomarker candidates in biliary tract cancer (BTC) using serum proteome analysis. Among several candidates, we focused on thrombin light chain which is a 4204 Da peptide as the most promising biomarker for BTC. To move thrombin light chain toward potential diagnostic use, we developed an enzyme immunoassay that enables to measure serum thrombin light chain levels....

BACKGROUND House dust mite (HDM) allergen quantification in house dust samples before and after the allergen elimination is one means of convincing the target population about the health benefits of allergen removal from their environment. OBJECTIVE To produce local reagents for quantification of Der f 1 (major allergen of Dermatophagoides farinae) in dust samples from houses of HDM allergic ...