The male gametophyte of Arabidopsis is a three-celled pollen grain that is thought to contain almost all the mRNAs needed for germination and rapid pollen tube growth. We generated a reference map of the Arabidopsis mature pollen proteome by using multiple protein extraction techniques followed by 2-DE and ESI-MS/MS. We identified 135 distinct proteins from a total of 179 protein spots. We foun...

this study was conducted to determine the effect of cliniptilolite natural zeolite on deficit irrigation stress tolerance on maize cultivar ksc704. the experiment was laid out as a factorial design based on randomized complete block with three replications at seed and plant improvement institute, karaj in 2012. four irrigation levels, including irrigation after 70, 95, 120 and 145 mm cumulative...

Successful haploid induction in loquat (Eriobotrya japonica (Thunb.) Lindl.) through in situ-induced parthenogenesis with gamma-ray irradiated pollen has been achieved. Female flowers of cultivar 'Algerie' were pollinated using pollen of cultivars 'Changhong-3', 'Cox' and 'Saval Brasil' irradiated with two doses of gamma rays, 150 and 300 Gy. The fruits were harvested 90, 105 and 120 days after...

Single pollen grain polymerase chain reaction (PCR) has succeeded in several species, however only limited numbers of pollen grains were involved due to difficulties in pollen isolation and lysis. This has limited its application in genetic analysis and mapping studies in plants. A high-throughput (HT) procedure for collecting and detecting genetic variation in a large number of individual poll...

Major genes govern the fertilization of teosinte ovules by maize pollen. A pollen-pistil compatibility system different from the previously described systems, Ga1-s and Tcb1-s, was identified among maize lines introgressed with chromosome segments from 2 teosinte populations. The pistil barrier is dominant, and pollen competence is determined by genotype of the individual pollen grain. A major ...

This investigation was done in order to study the response of grain yield and yield components of bread wheat cultivars to drought stress in western Iran in 2008-2009. The main plots were levels of drought stress (full irrigation, stress at early stem elongation, flowering and grain filling stages) and subplots were five cultivars (Chamran, Dez and Verinak). The results indicated that the effec...

this study was performed in order to evaluate the effects of drought stress and nitrogen fertilizer on phenology, morphology and yield of four cultivars of chickpea. this experiment was performed in a split-factorial using randomized complete block design with three replications. drought stress treatment stood in main plots (in three levels) consisting of no drought stress (control), moderate d...

Traditional pollination of date palms is a time-consuming and labor-intensive process. Hence, the objectives this study were 1) to develop rapid, efficient, low-cost method that results in an acceptable level fruit set (FS) with smaller amount pollen grains, labor costs using platform-mounted robotic (drone) supplemented water-suspended 2) investigate effect on palm FS percentage, efficiency (P...