OBJECTIVE To compare PCR (Polymerase Chain Reaction) with blood culture, typhi-dot and Widal test for the diagnosis of typhoid in patients taking antibiotics. STUDY DESIGN Cross-sectional, comparative study. PLACE AND DURATION OF STUDY National University of Sciences and Technology, Islamabad, Pakistan, from April 2013 to August 2014. METHODOLOGY One hundred and five patients were include...

Over the past five years 25,000 blood cultures were screened for septicaemia. Of these 6.7% were positive for salmonella species, the commonest being salmonella typhi accounting for 92% of total salmonella isolates. Over the years, S. typhi showed a gradual decline ranging from 96.7 to 87%. While salmonella paratyphi A has shown a gradual increase ranging from 2.5% to 11.4%. Salmonellosis peake...

Raw sequence data representing the majority of a bacterial genome can be obtained at a tiny fraction of the cost of a completed sequence. To demonstrate the utility of such a resource, 870 single-stranded M13 clones were sequenced from a shotgun library of the Salmonella typhi Ty2 genome. The sequence reads averaged over 400 bases and sampled the genome with an average spacing of once every 5,0...

BACKGROUND Salmonella serovars Typhi (S. Typhi) and Paratyphi A (S. Paratyphi A), the causative agents of enteric fever, have been routinely isolated organisms from the blood of febrile patients in the Kathmandu Valley since the early 1990s. Susceptibility against commonly used antimicrobials for treating enteric fever has gradually changed throughout South Asia since this time, posing serious ...

INTRODUCTION It is important to identify Salmonella Typhi infection quickly to treat acute fever patients and to prevent transmission by chronic typhoid carriers; therefore, a very specific and sensitive diagnostic technique is highly desirable, especially in endemic areas. The objective of this study was to develop a PCR protocol targeting the putative fimbrial staA gene of S. Typhi. This is a...

BACKGROUND The Malawi Liverpool Wellcome Trust Clinical Research Programme (MLW) has routinely collected specimens for blood culture from febrile patients, and cerebrospinal fluid from patients with suspected meningitis, presenting to Queen Elizabeth Central Hospital (QECH), Blantyre, Malawi, since 1998. METHODS We present bloodstream infection (BSI) and meningitis surveillance data from 1998...

recognition of typhoid hepatitis is important since it has to be differentiated from other common ailments in our country such as viral, malarial or amoebic hepatitis. early institution of specific therapy in cases of typhoid hepatitis carries a good prognosis. in our study, 107 patients with positive blood or bone marrow cultures for salmonella typhi, were evaluated for hepatomegaly and abnorm...

chemical precipitation method was used in order to synthesize cdse:ag quantum dots (2-3 nm). their physical properties and characteristics were assessed by x-ray diffraction, ultra violet-visible spectrophotometer and tem (transmission electron microscope) and it was shown that the obtained cdse:ag quantum dots are cubic with high-quality. antibacterial effects of cdse:ag nanoparticles against ...

ISSN: 2319-7706 Volume 5 Number 4 (2016) pp. 803-810 Journal homepage: http://www.ijcmas.com Salmonella typhi has been the most common aetiological agent of enteric fever syndrome while S. paratyphi A is responsible for only a minority of enteric fever cases. Salmonella typhi has also rapidly gained resistance to antibiotics like ampicillin, chloramphenicol and cotrimoxazole, and also to previo...