An artificial rumen for the study of nutrient digestion by the micro-organisms of the rumen was initially developed by Louw, Williams & Maynard (1949). In this, the micro-organisms were contained in a semi-permeable dialysis sac and the diffusible products of digestion removed by a salt solution, the composition of which approximated to that of saliva and which flowed continuously past the outs...

The relative contributions to O2 consumption made by the protozoal and bacterial populations present within the rumen were determined by using an open-type oxygen electrode system. Measurements indicated that two separate microbial populations contributed approximately equally to ruminal O2 consumption over the O2 concentration range experienced in situ (0.25 to 1.0 microM). The populations wer...

Many studies have shown the beneficial effects on ruminant performance of feeding them with silages inoculated with lactic acid bacteria (LAB). These benefits might derive from probiotic effects. The purpose of the current study was to determine whether LAB included in inoculants for silage can survive in rumen fluid (RF), as the first step in studying their probiotic effects. Experiments were ...

The suggestion that micro-organisms are responsible for the decomposition of cellulose in the alimentary canal (Wildt, 1874; Zuntz, 1879; Duclaux, 1882) arose from the proof (Henneberg & Stohmann, 1860-4) that it is, in fact, cellulose which disappears during the pa#sage of crude fibre through the animal (Haubner, 1855). Tappeiner (1882, 1883, 1884, 1888), who was the first to investigate the f...

In vitro fermentations of pure cellulose by mixed ruminal microorganisms were conducted under conditions in which pH declined within ranges similar to those observed in the rumen. At low cellulose concentrations (12.5 g/L), the first-order rate constants (k) of cellulose disappearance were successively lower at initial pH values of 6.86, 6.56, and 6.02, but in each case the value of k was maint...

BACKGROUND Many microorganisms in midgut of mosquito challenge with their host and also other pathogens present in midgut. The aim of this study was presence of non-pathogens microorganisms like fungal flora which may be crucial on interaction between vectors and pathogens. METHODS Different populations of Anopheles stephensi were reared in insectary and objected to determine fungal flora in ...

D IGESTION experiments with cattle fed simplified rations produced results in this laboratory indicating that efficiency of roughage dry matter digestion was dependent upon the presence or absence of certain ration nutrients or feed components needed by rumen microorganisms. The nutrients or feed components which favorably influenced roughage digestion in cattle experiments were protein (N X6.~...

The importance of protein quality for practical rations becomes evident as soon as an attempt is made to economize and to establish for such rations the lowest level of protein that will give efficient production. Woodman & Evans (195 I) demonstrated this point with pigs fed on a simplified barley-fine-bran ration. Supplementation with fish meal at the rate of 4.2% protein gave efficient produc...

The effects of disodium fumarate on microbial growth, CH4 production and fermentation of three diets differing in their forage content (800, 500 and 200 g/kg DM) by rumen micro-organisms in vitro were studied using batch cultures. Rumen contents were collected from four Merino sheep. Disodium fumarate was added to the incubation bottles to achieve final concentrations of 0, 4 and 8 mm-fumarate,...

Anaerobic rumen microorganisms mainly bacteria, protozoa and fungi degrade ligno-cellulosic feeds consumed by the ruminants. The ruminants in developing countries are predominantly maintained on low grade roughage and grazing on degraded range land resulting in their poor nutrient utilization and productivity. Hence, manipulation of rumen fermentation was tried during last two decades to optimi...