the aim of this study was to evaluate the effect of substitution of soybean meal with canola meal and measure its effects on rumen anaerobic fungal population in lactation iranian of holstein cows. from d 5 to 56 postpartum, cows were fed diets that were isoenergetic containing soybean meal (sbm; n = 3) or canola meal (cm; n = 3). cows were housed in tie stalls and fed tmr two times a day. comp...

The ecology of the uncultured, but large and morphologically conspicuous, rumen bacterium Oscillospira spp. was studied. Oscillospira-specific 16S rRNA gene sequences were detected in North American domestic cattle, sheep from Australia and Japan, and Norwegian reindeer. Phylogenetic analysis of the sequences obtained allowed definition of three operational taxonomic units within the Oscillospi...

Ruminants have a unique ability to derive energy from the degradation of plant polysaccharides through the activity of the rumen microbiota. Although this process is well studied in vitro, knowledge gaps remain regarding the relative contribution of the microbiota members and enzymes in vivo. The present study used RNA-sequencing to reveal both the expression of genes encoding carbohydrate-acti...

Herbivores as well as other vertebrates are not capable of producing cellulases and/or hemicelullases. Instead, a lot of herbivores form symbiotic associations with bacteria, protozoa and fungi which produce these enszymes and therefore are capable of degrading the plant polymers. In return for the relatively constant environment and continuous flow of plant material, the microorganisms of the ...

A combination of Sanger and 454 sequences of small subunit rRNA loci were used to interrogate microbial diversity in the bovine rumen of 12 cows consuming a forage diet. Observed bacterial species richness, based on the V1-V3 region of the 16S rRNA gene, was between 1,903 to 2,432 species-level operational taxonomic units (OTUs) when 5,520 reads were sampled per animal. Eighty percent of specie...

To assess the relative contributions of microbial groups (bacteria, protozoa, and fungi) in rumen fluids to the overall process of plant cell wall digestion in the rumen, representatives of these groups were selected by physical and chemical treatments of whole rumen fluid and used to construct an artificial rumen ecosystem. Physical treatments involved homogenization, centrifugation, filtratio...

The rumen microbiome represents a complex microbial genetic web where bacteria, anaerobic rumen fungi (ARF), protozoa and archaea work in harmony contributing to the health and productivity of ruminants. We hypothesized that the rumen microbiome shifts as the dairy cow advances in lactations and these microbial changes may contribute to differences in productivity between primiparous (first lac...

Management of metabolic hydrogen ([H]) in the rumen has been identified as an important consideration when reducing ruminant CH4 emissions. However, little is known about hydrogen flux and microbial rumen population responses to CH4 inhibition when animals are fed with slowly degradable diets. The effects of the anti-methanogenic compound, chloroform, on rumen fermentation, microbial ecology, a...

this study was carried out to determine the chemical composition and degradability parameters of dm, adf and ndf in alnus subcordata saw dust (sd) biodegraded by phanerochaete chrysosporium and pleurotus ulmarius fungi. the sd samples were incubated by the fungi at 30 ± 1˚c and 65 ± 5% relative humidity for 7 and 17 days. biodegraded samples were sterilized to constant activity and oven dried f...