In the present study, the effects of different forage-to-concentrate ratios (F:C) and an alkyl polyglycoside (APG) supplementation on parameters of rumen and blood metabolism were investigated in goats. A 2 x 2 factorial experiment was arranged within a 4 x 4 Latin square design (four 22-day periods), using four wether goats equipped with permanent ruminal cannulas. The experimental diets inclu...

The bacterium Actinobacillus succinogenes GXAS137, an efficient producer of succinic acid, was isolated from bovine rumen in Nanning, Guangxi Province, China. Here, we present the 2.3-Mb genome assembly of this strain, which consists of 2,314,479 bp (G+C content of 44.89%) with a circular chromosome, 2,235 DNA coding sequences, 57 tRNAs, and 15 rRNAs.

rumen is a persistent and specific ecosystem consists of bacteria, protozoa and fungus where feed fermentation takes place in it. produced hydrogen in rumen can be used in the synthesis of the volatile fatty acids and the microbial protein and its excess would be eliminated through the production of methane by methanogenesis. nutritionists have tried to find ways to decrease loss and energy and...

The Effects of using Acid-Consuming Bacteria and Different Buffers with High Concentration Diets on In Vitro Digestion Fermentation Activity Anaerobic Fungi Fattening Lambs Rumen

OBJECTIVE This experiment was conducted to explore the impact of diet on the ruminal microbial community in goats. METHODS Twelve goats were divided into two groups and fed complete feed (CF) or all forage (AF) diet. The total microbial DNAs in the rumen liquid were extracted. The V4 region of microbial 16S rRNA genes was amplified and sequenced using high-throughput. Information of sequences...

I . A technique for the separation and colorimetric estimation of z,4-diaminopimelic acid (DAP) using automated ion-exchange chromatography coupled with an acid ninhydrin detection system is described. 2. Only traces of DAP were found in rumen protozoa and no DAP was detected in rumen fluid prepared by ultracentrifugation or dialysis. 3. The concentration of DAP in rumen bacteria from sheep on ...

Subacute Ruminal Acidosis (SARA) corresponds to an imbalance between lactate-producing bacteria and lactate-using bacteria, which results in a change in ruminal pH associated with a prevalent consumption of rapidly fermentable carbohydrates. In our study, 216 primiparus and multiparus dairy cows were selected from 20 Italian intensive dairy herds and were divided into three groups based on the ...

Feeding grain to animals not adapted to grain resulted in a marked increase in the . / . concentration of free endotoxln m the rumen. Endotoxin concentration increased 15 to 18 times within 12 hr after lactic acidosis was induced through grain engorgement. The increase was accompanied by a shift from predominantly gram-negative to gram-positive bacteria. Data from in vitro fermentations showed ...

Rumen microbes produce cellular protein inefficiently partly because they do not direct all ATP toward growth. They direct some ATP toward maintenance functions, as long-recognized, but they also direct ATP toward reserve carbohydrate synthesis and energy spilling (futile cycles that dissipate heat). Rumen microbes expend ATP by vacillating between (1) accumulation of reserve carbohydrate after...

This paper describes the development of a microbial fuel cell (MFC) for electricity production from cellulose that is based on mixed culture of rumen bacteria in vitro. The fibrolytic bacteria isolated from rumen fluid were used in anode compartment of MFC, which can release glucose up to 15 g/L from carboxymethyl cellulose (CMC). At the same time, the maximum power density of 100.60mWm was gen...