"Bead beating" is commonly used to release DNA from cells for genomic studies but it was used here to prepare suspensions of plant nuclei for measurement of DNA amounts by flow cytometry. Plant material was placed in 2-ml screw-capped tubes containing beads of zirconia/silica (2.5 mm diameter) or glass (2.5 or 1.0 mm diameter) and 1 ml of lysis buffer. The tubes were mechanically shaken with an...

It was during the 1950's that the development of flow cytometry started, technology that allow to measure physiochemical characteristics of cells or suspended particles in fluid. This technology uses monoclonal antibodies labeled by fluorochromes as investigation tool in several analysis and needs isotype controls to define the negative region (background). These controls are constituted by imm...

Rosa canina belongs to rosacea family. Rosa. has a high concentration of phyto-constituents such as flavonoids, carotenoids, triterpene and vitamins vitamin C, E, A have an anti-inflammatory antioxidant
 
 effects. The antioxidant effect presence large quantities phytochemicals flavonoids polyphenols. been used for arthritis gout, osteoarthritis, urinary tract disorder, diabetes, infl...

Rosehip is a good source of some biologically active compounds. The main importance of rosehip is its high composition of vitamin C and rutin (vitamin P) and high content of pectins, fatty acids, sugars, organic acids, phenolic components, lycopene, carotene with activity of vitamin A and group B vitamins, tannins, carotenoids, minerals (particularly K and P) macroand microelements (Tuer & Russ...