RNA synthesis in Sarcoma 180 ascites cells growing in vivo in mice was compared to RNA synthesis in Sarcoma 180 cells following transfer to synthetic medium. Following radioactive labeling of Sarcoma 180 cells, RNA was extracted with phenol and fractionated on methylated bovine serum albumin columns. The cells appeared to experience a nutritional shift with resulting cessation of ribosomal RNA ...

RNA synthesis in Sarcoma 180 ascites cells growing in vivo in mice was compared to RNA synthesis in Sarcoma 180 cells following transfer to synthetic medium. Following radioactive labeling of Sarcoma 180 cells, RNA was extracted with phenol and fractionated on methylated bovine serum albumin columns. The cells appeared to experience a nutritional shift with resulting cessation of ribosomal RNA ...

Using fluorescence resonance energy transfer, we show that, in the majority of transcription complexes, sigma(70) is not released from RNA polymerase upon transition from initiation to elongation, but, instead, remains associated with RNA polymerase and translocates with RNA polymerase. The results argue against the presumption that there are necessary subunit-composition differences, and corre...

isomorphous replacements, for clarification and possible decision among the several structures proposed. tRNA has long been known to be rich in minor base modifications (6, 20), of which the most common is methylation, and this occurs on all 4 major bases. Data presented at this symposium by Stulberg and Shugart (72) support the logical inference that these base modifications change the tertiar...

The mitochondria of yeast,1 Neurospora,2 and Tetrahymena3 have been shown to contain transfer RNA (tRNA). Barnett et al.4 have found, in addition, that Neurospora mitochondria contain 15 aminoacyl-tRNA synthetases, at least three of which acylate only mitochondrial tRNA. The purpose of this communication is twofold. First we will present evidence showing that, as with eukaryotic microbial syste...

The role of the nucleolus in the transfer of RNA from nucleus to cytoplasm was examined by means of experiments in which the nucleolus or other parts of the nucleus were inactivated by a microbeam of ultraviolet light. These experiments confirmed previous work in showing that such inactivation of the nucleolus inhibited the appearance in the cytoplasm, not only of ribosomal RNA, but also of oth...