OBJECTIVE to quantify placenta-specific RNA in plasma of women carrying foetuses with intrauterine growth restriction and pregnant women with normal pregnancies. METHODS 8 pregnant women with foetuses with intrauterine growth restriction were studied as well as 18 women with uncomplicated pregnancies in the third pregnancy trimester. Total free RNA was quantified in maternal plasma by spectro...

Transport of messenger RNA (mRNA) from the nucleus to the cytoplasm is an essential step of eukaryotic gene expression. In the cell nucleus, a precursor mRNA undergoes a series of processing steps, including capping at the 5' ends, splicing and cleavage/polyadenylation at the 3' ends. During this process, the mRNA associates with a wide variety of proteins, forming a messenger ribonucleoprotein...

Previous studies have revealed a loss of enkephalin immunoreactivity in the terminals of striatal neurons projecting to the external globus pallidus in patients with early grades of Huntington’s disease (HD). To asess the status of the petikarya of striatal enkephalinergic neurons, we performed in situ hybridization histochemistry with a radiolabeled RNA probe complementary to preproenkephalin ...

Messenger RNA transport was studied in KB cells infected with the nuclear DNA virus adenovirus type 2. Addition of 0.04 microg/ml of actinomycin completes the inhibition of ribosome synthesis normally observed late after infection and apparently does not alter the pattern of viral RNA synthesis: Hybridization-inhibition experiments indicate that similar viral RNA sequences are transcribed in ce...

SUMMARY The influence of cytoplasmic factors on the energy-dc pendent release from nuclei of messenger-like RNA in ribonucleoprotein particles (i.e., informosomes) has been investigated in a cell-free system that precludes detectable nuclear lysis. Maximum release of rapidly labeled RNA from the prelabeled nuclei of normal, regenerating, or neo plastic rat liver was observed only when the latte...

Ongoing research into the use of messenger RNA (mRNA) vaccines for treatment cancer has been expediated by coronavirus pandemic because similar technology was used in development mRNA COVID‐19 vaccines. So how close are we now to widespread clinical anti‐cancer vaccines?

It is difficult to believe that only 2 years ago, the very existence of messenger RNAs in animal cells was doubtful, and no definitive example of an isolated and purified mRNA could be given. Since that time, the mRNAs for globin (from rabbit, man, mouse, duck, and guinea pig), lens crystallin (chick and cow), ovalbumin, myosin, and silk fibroin have been isolated and their identities confirmed...

Multiple gene silencing is being required to target and tangle metabolic pathways in eukaryotes and researchers have to develop a subtle method for construction of RNA interference (RNAi) cassettes. Although, several vectors have been developed due to different screening and cloning strategies but still some potential limitations remain to be dissolved. Here, we worked out a simple cloning stra...

Escherichia coli ribonuclease III cleaves adenovirus messenger RNA and mammalian 28S and 18S ribosomal RNA. Fragmentation is not random, but in each case a specific collection of products is generated. This points to the potential use of the enzyme as a tool for specific fragmentation of RNA. Cleavage by RNase III abolishes the capability of adenovirus messenger RNA to direct cell-free synthesi...

Late adenovirus messenger RNA was annealed to complementary regions of partially melted viral double-stranded DNA. The RNA. DNA hybrid regions within the DNA molecules were visualized as loops in the electron microscope. Loops occurred at several regions of the DNA, most frequently, however, at a location near the center of the molecule. This hybridization technique appears well suited for an a...