Abstract Background Heart failure (HF) coincides with cardiomyocyte telomere shortening. Arterial hypertension is the most prominent risk factor for HF. Both HF and arterial are associated dysregulation of neurohormonal axis increased ROS. However, how activation linked to shortening in pathogenesis incompletely understood. Further, recent findings suggest that peroxiredoxin 1 (PRDX1) can act a...

Abstract α-thalassaemia/mental retardation X-linked (ATRX) mutations are a critical molecular marker for high-grade glioma (HGG). These lead to accumulations of abnormal DNA secondary G-quadruplex (G4) structures, thereby inducing replication stress and damage. As G4s arise at GC-rich regions (i.e., pericentromeric telomeric regions), ATRX-deficiency alters genome-wide accessibility chromatin c...

Damage repair mechanisms at transcriptionally active sites during the G0/G1 phase are largely unknown. To elucidate these mechanisms, we introduced genome site-specific oxidative DNA damage and determined the role of transcription in repair factor assembly. We find that KU and NBS1 are recruited to damage sites independent of transcription. However, assembly of RPA1, RAD51C, RAD51, and RAD52 at...

Synthesis of ribosomal RNA by RNA polymerase I (RNA pol I) is an elemental biological process and is key for cellular homeostasis. In a forward genetic screen in C. elegans designed to identify DNA damage-response factors, we isolated a point mutation of RNA pol I, rpoa-2(op259), that leads to altered rRNA synthesis and a concomitant resistance to ionizing radiation (IR)-induced germ cell apopt...

Background: The varicocele (VCL) impacts the testicular tissue both by enhancing tissue inflammation and by down-regulating the antioxidant status. On the other hand, in varicocele patient the critical role of Hsp70 in spermiogenesis is identified. Therefore present study was estimated to evaluate the protective effect of testosterone and vitamin E on VCL-decreased Hsp70 expression and on VCL-i...

RNA sequencing (RNA-seq) technology has now become one of the standard tools for studying biological mechanisms at transcriptome level. Advances in RNA-seq have led to emergence a large number publicly available data analysis. Most them target linear genome sequences although it is necessary study organisms with circular sequences. For example, by infection viroids which comprise 246–401...

Ethanol and Reactive Species Increase Basal Sequence Heterogeneity of Hepatitis C Virus and Produces Variants With Reduced Susceptibility To Antivirals Jinah Choi, Scott Seronello, Jessica Montanez, Kristen Presleigh, Thu Vo, Seung Bum Park, and Miriam Barlow University of California, Merced Hepatitis C virus exhibits a high level of genetic variability, and variants with reduced susceptibility...

Hepatitis C virus (HCV) exhibits a high level of genetic variability, and variants with reduced susceptibility to antivirals can occur even before treatment begins. In addition, alcohol decreases efficacy of antiviral therapy and increases sequence heterogeneity of HCV RNA but how ethanol affects HCV sequence is unknown. Ethanol metabolism and HCV infection increase the level of reactive specie...