The Gram-positive bacteria Rhodococcus opacus ISO-5 and Rhodococcus sp. RHA1 utilized taurine (2-aminoethanesulfonate) as the sole source of carbon or of nitrogen or of sulfur for growth. Different gene clusters and enzymes were active under these different metabolic situations. Under carbon- or nitrogen-limited conditions three enzymes were induced, though to different levels: taurine-pyruvate...

BACKGROUND Cholesterol oxidase (CHO) has various clinical and industrial applications. Recently, microbial CHO have received a great attention for their wide usage in medicine. Here, taxonomic characterizations of isolated strain from soil, optimization of the conditions for CHO production and biochemical characterizations of produced CHO enzyme were described. Finally, CHO gene was cloned into...

A methodology is presented for calculating of the surface potential, Donnan potential, and ion concentration profiles for semipermeable microbial membranes that is valid for an arbitrary electrolyte composition. This model for surface potential, Donnan potential, and charge density was applied to recently reported experimental data for gram-positive bacteria, including Bacillus brevis, Rhodococ...

The genus Rhodococcus is gram positive bacterium which was classified into the family of Nocardiaceae.  The organism can be isolates from different sources, with capability to degrade diverse types of pollutant. Nowadays, scientists reported a few secondary metabolites which are produced by Rhodococci. Hence, the present study tried to isolate Rhodococci from different soil samples with the cap...

Rhodococcus is an emerging cause of opportunistic infection in immunocompromised patients, most commonly causing cavitary pneumonia. It has rarely been reported as a cause of isolated bacteremia. However, the relationship between bacteremia and central venous catheter is unknown. Between 2002 and 2010, the characteristics and outcomes of seventeen cancer patients with Rhodococcus bacteremia and...

Oligopeptidase B (OPB; EC 3.4.21.83) from 2 Gram-negative bacteria, Stenotrophomonas maltophilia (Stm) and Serratia marcescens (Sem), and the Gram-positive bacterium Rhodococcus erythropolis (Re) were cloned and characterized to clarify their activities and substrate specificities using peptidyl-MCA substrates containing Arg or Lys. The cloned enzymes, Stm, Sem and ReOPBs, in addition to Escher...

A Gram-negative bacterial isolate, named PG4, was recovered from the rhizosphere of a tobacco plant (Nicotiana tabacum cv. Burley) and was identified as Lysobacter capsici based on the sequencing and phylogenetic analysis of 16S rRNA and characteristics in the Biolog Identification System. Strain PG4 showed chitinase and protease activity and was able to inhibit the growth of a broad range of p...

Four different bacteria capable of degrading butachlor, as well five syntrophic pairs able to break down were isolated from rice paddy soils in Korea. Genetic and phenotypic analyses conducted better understand their characteristics behavior. All single isolates utilize butachlor a sole carbon energy source. Analysis the 16S rRNA sequence showed that related members genus Rhodococcus new type b...

A total of 2318 heterotrophic nitrogen removal (HNR) bacteria isolated from piggery wastes (after biogas container) were classified in four kinds of heterotrophic ammonia-oxidizing bacteria (569 isolates), nitrite-oxidizing bacteria (580 isolates), nitrate-oxidizing bacteria (600 isolates) and heterotrophic nitrifying and denitrifying bacteria (569 isolates). The virtually complete 16S rRNA gen...