Catalysts are widely used in many industries for production of chemicals, pharmaceuticals, fuels and energy. There is a need for more sustainable production processes, with reduced energy and raw material consumption together with a reduction in waste and toxic by-products. Conventional synthetic catalysts often demand harsh chemical conditions and multi-step processes. Biocatalysts, i.e., enzy...

Background: Lipase enzymes are of great importance in various industries. Currently, extensive efforts have been focused on exploring new lipase producer microorganism as well as genetic and protein engineering of available lipases to improve their functional features. Methods: For screening lipase-producing lactobacilli, isolated strains were inoculated onto tributyrin agar plates. Molecular ...

Asperfuran is a novel antifungal dihydrobenzofuran derivative produced by a strain of Aspergillus oryzae. Asperfuran weakly inhibited chitin synthase from Coprinus cinereus. This inhibition could be abolished by the addition of egg lecithin. In the agar diffusion assay asperfuran induced morphological changes in Mucor miehei at very low concentrations (20 ng/disc) while growth was only partly i...

Cloning and sequencing of a lipase gene from an indigenous Bacillus pumilus, strain F3, revealed an open-reading frame of 648 nucleotides predicted to encode a protein of 215 residues. Sequence analysis showed that F3 lipase contained a signal peptide composed of 34 amino acids with an H domain of 18 residues. A tat-like motif was found in the signal peptide similar to some other Bacillus pumil...

In this study, solid-state fermentation of two types of agricultural residues/products for lipase production in a tray-bioreactor was investigated. Rhizopus oryzae was used as a potential fungus strain and two types of agricultural residues including soybean meal and sugarcane bagasse were utilized as substrate. Fermentation was carried out in two different operational conditions: one with cont...

In the current study, thermo-tolerant fungal isolates were screened for their ability to metabolize L-methionine by γ-lyase (MGL). Among 63 isolates, fifteen exhibited MGL induction as evidenced qualitative assay method using phenol red. Then, quantitative indicates that coded 26 & 37 most potent production. Fungal mycelium treatment under sonication process proved isolate No. could be release ...

milk clotting enzyme (m ce) produced by: rhizomucor miehei was purified and characterized.the enzyme was purified 220.29-fold with specific activity about 14444.2 u/mg protein byultrafiltration, ammonium sulfate fractionation, sephacryl s-300 chromatography. the maximumenzyme activity was at 65°c.the milk clotting activity was decreased steadily as ph is increased and indicated maximumactivity ...

background: lipase is an enzyme with immense application potential. ester synthesis by lipase catalysis in organic media is an area of key industrial relevance. enzymatic preparations with traits that cater to the needs of this function are hence being intensely researched. objective: the objectives of the study were to immobilize the lipase from bacillus sp. ps35 by cross-linking and adsorptio...

in this study, the effect of addition of three different levels of microbial lipase (0, 4, and 8.0 g lipase in 100 kg milk) was investigated on the physiochemical properties, free fatty acids, textural properties, and microstructure of lighvan cheese during ripening. the addition of lipase did not significantly affect ph, acidity, moisture, and salt content of lighvan cheese, but increased its ...