BACKGROUND Toxoplasma gondii is one of the most common causes of latent infections in humans worldwide. Detecting anti-Toxoplasma antibodies in serum using serological tests is a common method to diagnose toxoplasmosis. OBJECTIVES In the present study, an indigenous ELISA kit was prepared using tachyzoites from the RH strain of T. gondii, and its sensitivity and specificity were compared with...

Moran, Daniel S., Yair Shapiro, Arie Laor, Sharona Izraeli, and Kent B. Pandolf. Can gender differences during exercise-heat stress be assessed by the physiological strain index? Am. J. Physiol. 276 (Regulatory Integrative Comp. Physiol. 45): R1798–R1804, 1999.—A physiological strain index (PSI) based on rectal temperature (Tre) and heart rate (HR) was recently suggested to evaluate exercise-he...

toxoplasma gondii contain various immunogenic antigens. the most important toxoplasma antigens are somatic and excreted/secreted antigens. rhoptry proteins are known as excreted/secreted antigens. these antigens have been proposed as a vaccine candidate against toxoplasmosis. the main objective of the present work was cloning rhoptry protein1 (rop1) gene of toxoplasma gondii (rh) in a cloning v...

The effect of relative humidity (RH) on the survival of airborne viruses is not uniform. Certain airborne viruses, measles and influenza, for example, survive best when held at low RH values (5, 9). The picornaviruses and adenoviruses, on the other hand, exhibit greatest survival in the airborne state at high RH values (8, 10); at least one virus, pigeon pox, survives quite well regardless of t...

BACKGROUND The aim of this study was to evaluate the effects of conjugated linoleic acid (CLA) on apoptosis of tachyzoites of T. gondii, RH strain (type I) and the cyst-forming Tehran strain (type II) in vitro. METHODS Toxoplasma strains were injected into the peritoneal cavity of BALB/c mice. The Tehran strain forms cysts in the brain of mice. Bradyzoites within the cysts are reactivated to ...

We examined the effect of short-term heat acclimation with permissive dehydration (STHADe) on heat acclimation (HA) and cycling performance in a temperate environment. Ten trained male cyclists [mean (SD) maximal oxygen uptake: 63.3(4.0) mL/kg/min; peak power output (PPO): 385(40) W; training: 10 (3) h/week] underwent a STHADe program consisting of 5 days of exercise (maximum 90 min/day) in a h...

Recent multistate outbreaks of salmonellosis associated with fresh cucumbers underscore the importance understanding Salmonella behavior on under different conditions. This study developed mathematical models to predict survival four-strain cocktail whole at temperature and relative humidity (RH) The strains were Newport H1275 Stanley H0558 (sprout outbreaks), Montevideo G4639 (tomato outbreak)...

Ketolides are a new class of macrolide antibiotics that have been shown to be active against a variety of bacteria including macrolide-resistant bacteria and mycobacteria. We examined two ketolides, HMR 3647 and HMR 3004, for their in vitro and in vivo activities against the protozoan parasite Toxoplasma gondii. In vitro, both ketolides at concentrations as low as 0.05 microg/ml markedly inhibi...

Neospora caninum is a protozoan parasite that can cause severe disease in mammals. Two experiments were conducted to examine the effects of subcutaneous (s.c.) vaccination with Hank's balanced salt solution (HBSS), 1 x 10(5) N. caninum NC-1 strain tachyzoites or 1 x 10(5) Toxoplasma gondii TS-4 strain tachyzoites on challenge oral infections in mice with sporulated VEG strain T. gondii oocysts ...

A physiological strain index (PSI), based on rectal temperature (Tre) and heart rate (HR), was recently suggested for evaluating heat stress. The purpose of this study was to evaluate the PSI for different combinations of hydration level and exercise intensity. This index was applied to two databases. The first database was obtained from eight endurance-trained men dehydrated to four different ...