BACKGROUND Animal mycobacterioses are regarded as a potential zoonotic risk and cause economical losses world wide. M. avium subsp. hominissuis is a slow-growing subspecies found in mycobacterial infected humans and pigs and therefore rapid and discriminatory typing methods are needed for epidemiological studies. The genetic similarity of M. avium subsp. hominissuis from human and porcine origi...

BACKGROUND Linear dermatitis is endemic in Iran where most cases occur in the Caspian Sea coast and Fars province. The disease is caused by beetles of the genus Paederus which are active from early spring to beginning of autumn although its incidence rises from May to August. The classic taxonomy of Paederus spp. is based on the male genitalia that is very complex and needs expertise. In this s...

Recent studies have shown the usefulness of PCR-based restriction fragment length polymorphism (RFLP) analysis for differentiating Helicobacter pylori strains isolated by culture. For this study, a PCR-based RFLP assay was developed for directly typing H. pylori strains from gastric biopsy specimens. Nineteen gastric biopsy specimens obtained from patients undergoing endoscopy for gastrointesti...

The combination of preventive vaccination and diagnostic typing of viral isolates from patients with clinical poliomyelitis constitutes our main protective shield against polioviruses. The restriction fragment length polymorphism (RFLP) adaptation of the reverse transcriptase (RT)-PCR methodology has advanced diagnostic genotyping of polioviruses, although further improvements are definitely ne...

Molecular epidemiology that integrates molecular approaches into the convention epidemiologic investigations has proved quite useful in tracing the transmission of drug resistant Mycobacterium tuberculosis in USA. Molecular epidemiological tools for tuberculosis include both restriction fragment length polymorphism (RFLP) as well as PCR methods. The most useful methods are the IS 6110 RFLP anal...

conclusions as about 70% of methicillin-resistant isolates belonged to sccmec iii, the present study can conclude that, over an 8-year period, only one dominant and stable clone of mrsa strain was found in tabriz hospitals. this finding could be a result of incorrect medical orientations, inadequate infection controlling policies, and insufficient preventive approaches. objectives the current s...

Calreticulin (CALR) mutations are included in the WHO diagnostic criteria of Myeloproliferative Neoplasm (MPN). Presence CALR mutation, aids their diagnosis by excluding reactive causes. There several molecular techniques to detect mutations; however, these require advanced equipment and technical expertise. An in-house “polymerase chain reaction-restriction fragment length polymorphism (PCR- R...

Restriction fragment length polymorphism (RFLP) typing of Mycobacterium tuberculosis is a recently developed technique which allows strains of tuberculosis to be differentiated with a high degree of confidence. The application of this technique has enabled the patterns of transmission of tuberculosis to be elucidated with far greater certainty than was previously possible. This review will disc...

background and objectives: molecular typing methods are important and useful tools to assess the transmission, diversity of strains and differentiation between new infections and relapses which can effectively help in controlling infections. the aim of this study was to evaluate the molecular typing methods which have been used in iran. by evaluating the results and discriminatory power of each...