نتایج جستجو برای: rflp pcr

تعداد نتایج: 177900  

Journal: :Parasitology 2002
M Gile D C Warhurst K A Webster D M West J A Marshall

A multiplex allele specific polymerase chain reaction (MAS-PCR) based on the Cryptosporidium parvum dihydrofolate reductase (dhfr) gene sequence differentiates genotype 1 ('Human') from 2 ('Cattle') in a 1-step reaction. The MAS-PCR was validated on a panel of 34 microscopically positive C. parvum faecal samples of human and animal origin in comparison with 2 published PCR-restriction fragment ...

2014
Anna Zaczek Anna Brzostek Arkadiusz Wojtasik Anna Sajduda Jaroslaw Dziadek

Fast and inexpensive identification of epidemiological links between limited number of Mycobacterium tuberculosis strains is required to initially evaluate hospital outbreaks, laboratory crosscontaminations, and family or small community transmissions. The ligation-mediated PCR methods (LM-PCR) appear sufficiently discriminative and reproducible to be considered as a good candidate for such ini...

2015
Vahhab Piranfar Malike Sharif Mojtaba Hashemi Ali Reza Vahdati Reza Mirnejad

OBJECTIVES Rapid and accurate detection of Brucella abortus and Brucella melitensis from clinical samples is so important because antibiotic treatment has major side effects. This study reveals a new method in detection of clinical samples of brucellosis using real-time PCR and high-resolution melt (HRM) curve analysis. MATERIALS AND METHODS 160 brucellosis suspicious samples with more than 1...

2014
Narges Cheraghchi Pejvak Khaki Soheila Moradi Bidhendi Azar Sabokbar

BACKGROUND Salmonella spp. is the major bacterial pathogen in poultry and is responsible for significant economic losses of the poultry industry in many parts of the world. Among Salmonella spp., Salmonella gallinarum and Salmonella. pullorum are the most common causative agents of chicken salmonellosis resulting in high mortality and morbidity. OBJECTIVES The aim of this study was to identif...

Journal: :Applied and environmental microbiology 2000
F Doignon-Bourcier A Willems R Coopman G Laguerre M Gillis P de Lajudie

We examined the genotypic diversity of 64 Bradyrhizobium strains isolated from nodules from 27 native leguminous plant species in Senegal (West Africa) belonging to the genera Abrus, Alysicarpus, Bryaspis, Chamaecrista, Cassia, Crotalaria, Desmodium, Eriosema, Indigofera, Moghania, Rhynchosia, Sesbania, Tephrosia, and Zornia, which play an ecological role and have agronomic potential in arid re...

ژورنال: :مجله دانشگاه علوم پزشکی کردستان 0
الناز اکبرزاده elnaz akbarzadeh master of microbiology, department of microbiology, islamic azad university, tehran north branch, tehran, iran.کارشناس ارشد میکروب شناسی، گروه میکروب شناسی، دانشگاه ازاد اسلامی واحد تهران شمال، تهران، ایران. جمیله نوروزی jamileh noroosi phd of medical microbiology, department of microbiology, islamic azad university, tehran north branch, tehran, iran.دکتری میکروب شناسی پزشکی، گروه میکروب شناسی، دانشگاه ازاد اسلامی واحد تهران شمال، تهران، ایران. وهاب پیرانفر vahab piranfar master of microbiology, molecular biology research center, baqiyatallah university of medical sciences, tehran, iran.کارشناس ارشد میکروب شناسی، مرکز تحقیقات بیولوژی مولکولی دانشگاه علوم پزشکی بقیه ا...(عج)، تهران، ایران. داریوش قاسمی dariush ghasemi master of medical biotechnology, molecular biology research center, baqiyatallah university of medical sciences, tehran, iran.کارشناس ارشد بیوتکنولوزی پزشکی، مرکز تحقیقات بیولوژی مولکولی دانشگاه علوم پزشکی بقیه ا...(عج)، تهران، ایران. شیوا میرکلانتری shiva mirkalantari phd of medical bacteriology, department of microbiology, school of medicine, semnan university of medical, semnan, iran.دکتری باکتری شناسی پزشکی، گروه میکروب شناسی، دانشکده پزشکی، دانشگاه علوم پزشکی سمنان، سمنان، ایران. رضا میرنژاد reza mirnejad phd of medical bacteriology, molecular biology research center, baqiyatallah university of medicalدکتری باکتری شناسی پزشکی، مرکز تحقیقات بیولوژی مولکولی دانشگاه علوم پزشکی بقیه ا...(عج)، تهران، ایران

مقدمه: مطالعات کمی در خصوص جداسازی بروسلا آبورتوس و بروسلا ملی تنسیس عامل بروسلوزیس از سرم بیماران انجام است، این مطالعه با هدف جداسازی همزمان بروسلاهای بیماری زا از نمونه های سرمی انسان به روش multiplex pcr طراحی گردید. روش ها: در این مطالعه آزمایشگاهی، 50 نمونه خون و سرم بیمار مشکوک با علائم بالینی بروسلوزیس، پس از تیترسنجی به محیط کشت بروسلا اگار تلقیح شده و برای تشخیص، از تکنیک مولکولی mult...

2002
Mukkamala Lakshmi Madasamy Parani Ajay Parida

Molecular phylogeny and genetic diversity in all the nine species (Rhizophora mucronata, R. apiculata, R. stylosa, Bruguira cylindrical, B. parviflora, B. gymnorriza, Ceriops tagal and C. decandra) and a natural Rhizophora hybrid, of the mangrove Rhizophoraceae, occurring in the Indian sub-continent were analysed using random amplified polymorphic DNA (RAPD), restriction fragment length polymor...

2018
Nova Zairina Lubis Kamaliah Muis Lukmanul Hakim Nasution

BACKGROUND Onychomycosis is a fungal infection of one or more units of the nail caused by dermatophytes, or mould and nondermatophytes yeast. Investigations are needed to establish the diagnosis of onychomycosis before starting treatment. Several investigations methods for diagnosing onychomycosis are microscopic examination with 20% KOH, fungal culture, histopathology examination with PAS stai...

2017
Mojgan ARYAEIPOUR Arezoo BOZORGOMID Bahram KAZEMI Masoud BEHNIA Hakim AZIZI Mohammad Bagher ROKNI

BACKGROUND We aimed to describe morphological and morphometrical characteristics of Fasciola spp. in livestock from Ardabil Province, Northwest Iran. METHODS Forty adult flukes were collected from different definitive hosts (cattle and sheep). Previously specimens were identified as F. hepatica or F. gigantica based on PCR-RFLP of the ITS-1 region with RsaI enzyme. We identified Fasciola spp....

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