نتایج جستجو برای: rflp method

تعداد نتایج: 1638664  

Journal: :Journal of clinical microbiology 2001
C Sola S Ferdinand C Mammina A Nastasi N Rastogi

In a previous study, we proposed to associate spoligotyping and typing with the variable number of tandem DNA repeats (VNTR) as an alternative strategy to IS6110-restriction fragment length polymorphism (RFLP) for molecular epidemiological studies on tuberculosis. The aim of the present study was to further evaluate this PCR-based typing strategy and to describe the population structure of Myco...

Journal: :iranian journal of biotechnology 2013
shima fayaz pezhman fard-esfahani shahnaz khaghani

background: the high resolution melting (hrm) method is a new scanning method for detecting unknown changes in dna and its advantages have persuaded researchers to recruit it as a screening method. objectives: here, we developed a hrm method to screen r188h snp (rs3218536) of xrcc2 and compared the results with a well known pcr-rflp technique. materials and methods: genomic dna samples from 350...

Journal: :Applied and environmental microbiology 2003
Delphine Paillard Véronique Dubois Robert Duran Fany Nathier Catherine Guittet Pierre Caumette Claudine Quentin

A molecular method based on restriction fragment length polymorphism (RFLP) of PCR-amplified fragments of the 23S rRNA gene was designed to rapidly identify Listeria strains to the species level. Two fragments (S1, 460 bp, and S2, 890 bp) were amplified from boiled DNA. S2 was cut with the restriction enzymes XmnI or CfoI and, if needed, S1 was digested by either AluI or ClaI. This method was f...

Journal: :Biomedica : revista del Instituto Nacional de Salud 2015
Julio-C Rendón Fabián Cortés-Mancera Alejandra Duque-Jaramillo Marta C Ospina María Cristina Navas

INTRODUCTION Ten viral genotypes (A-J) distributed in all continents have been described for hepatitis B virus (HBV). One of the methodologies for determining the viral genotype is the restriction fragment length polymorphism (RFLP) technique, a simple and relatively inexpensive method, albeit with some limitations. OBJECTIVE The initial objective of the project was to identify the HBV genoty...

2009
Cheong Ho Cho Sang Hoon Han Bum Sik Chin Suk Hoon Choi Han Sung Lee Chang Oh Kim Myung Soo Kim Jun Yong Choi Young Goo Song June Myung Kim

BACKGROUND/AIMS The development of effective, accurate, and rapid diagnostic methods for Mycobacterium infection and mycobacterial species identification is required. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) is an easy, rapid and inexpensive technique for identifying Mycobacterium spp. METHODS We performed PCR-RFLP to detect and identify Mycobacterium spp....

Journal: :Turkish journal of medical sciences 2014
Çağrı Şakalar Salih Kuk Ahmet Erensoy Adile Ferda Dağli İbrahim Hanifi Özercan Ülfet Çetınkaya Süleyman Yazar

BACKGROUND/AIM To develop a novel polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) protocol using a new genomic marker sequence and a novel set of restriction enzymes in order to detect and discriminate 2 Echinococcus species, E. granulosus and E. multilocularis, found in formalin-fixed paraffin-embedded (FFPE) human tissues. MATERIALS AND METHODS DNA was isolated...

Journal: :Acta medica Okayama 1998
S Inoue Y Yamamoto O Okamoto H Murakami S Miyaishi H Ishizu

A sensitive method of HLA-DRB1 typing was devised using a semi-nested polymerase chain reaction (PCR) followed by a restriction fragment length polymorphism (RFLP) analysis (semi-nested PCR-RFLP method). The first-round amplification (30 cycles) of the semi-nested PCR was performed using DRB generic primer pairs and the second round of PCRs (20 cycles) were performed using DRB1 group-specific p...

2014
K. Diba H. Mirhendi P. Kordbacheh S. Rezaie

In this study we attempted to modify the PCR-RFLP method using restriction enzyme MwoI for the identification of medically important Aspergillus species. Our subjects included nine standard Aspergillus species and 205 Aspergillus isolates of approved hospital acquired infections and hospital indoor sources. First of all, Aspergillus isolates were identified in the level of species by using morp...

2013
Hyeng-Il Cheun Kyungjin Kim Sejoung Yoon Won-Ja Lee Woo-Yoon Park Seobo Sim Jae-Ran Yu

There are approximately 20 known species of the genus Cryptosporidium, and among these, 8 infect immunocompetent or immunocompromised humans. C. hominis and C. parvum most commonly infect humans. Differentiating between them is important for evaluating potential sources of infection. We report here the development of a simple and accurate real-time PCR-based restriction fragment length polymorp...

Journal: :Investigative ophthalmology & visual science 1999
J Shimazaki M Kaido N Shinozaki S Shimmura B Munkhbat M Hagihara K Tsuji K Tsubota

PURPOSE Severe destruction of the corneal limbus causes conjunctival invasion and subsequent visual loss. Limbal allograft transplantation (LAT) was recently proposed for the treatment of these disorders. However, whether the method functions as a stem cell transplantation of the corneal epithelium remains unclear. This study provided evidence that donor-derived corneal epithelial cells survive...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید