One of the viral diseases affecting both domestic and wild animals in many countries around world is foot mouth disease (FMD). The livestock sector has been suffering significant financial losses for years as a result disease. present research work was focused on isolation, documentation serotyping Foot Mouth Disease (FMD) virus from different locations. Reverse Transcription Polymerase Chain R...

A PCR based reverse transcriptase (RT) assay was developed that has 10(4)-fold higher sensitivity than conventional nucleotide incorporation assays and allows discrimination between false positive results generated by cellular polymerases and positives resulting from authentic RT activity. Recently, several reverse transcriptase (RT) assays have been developed where a reverse transcriptase reac...

  Avian Influenza (AI) is a viral respiratory disease of domestic and wild birds. In the diagnostic laboratory, it is essential to have methods for rapid detection of avian respiratory viruses. Cloacal swabs collected from chickens experimentally infected with H9 subtype AI virus, used in a reverse transcription-polymerase chain reaction (RT-PCR) assay for detection of AI. In infected animals, ...

Human rhinoviruses (HRVs) are positive-stranded RNA viruses belonging to the Enterovirus genus in the family of Picornaviridae. Identification of the specific strain in HRV disease has been difficult because the traditional serological method is insensitive, labor intensive, and cumbersome. With the fast progress in molecular biological technique, more sensitive and faster molecular methods hav...

People's lives could be in danger if a contagious disease spreads quickly, Corona-2019 virus (COVID-19) is one. The coronavirus epidemic rapidly spread over the world. Corona has had major impact on health of populations and healthcare systems all RT-PCR (RT-Reverse transcription, PCR-polymerase chain reaction) testing can benefit from use computed tomography images. Most available methods larg...

Background: Severe acute respiratory syndrome corona virus 2 (SARS-CoV-2) transmission during laparoscopy while using energy devices through the carbon dioxide pneumoperitoneum is a big concern in operation theatre. So, we had to alter way vent closed-circuit system (CCS) avoid contamination.Methods: We did prospective study safety of CCS elective laparoscopic surgeries. recruited 184 patients ...

The coronavirus, which appeared in Wuhan city of China and named COVID-19 , spread rapidly caused the death many people. Early diagnosis is very important to prevent or slow spread. first preferred method by clinicians real-time reverse transcription-polymerase chain reaction (RT-PCR). However, expected accuracy values cannot be obtained patients incubation period. Therefore, common lung devast...