نتایج جستجو برای: reporter genes

تعداد نتایج: 446848  

Journal: :Integrative and comparative biology 2013
Antje H L Fischer Sarah Tulin David Fredman Joel Smith

Changes in the expression and function of genes drive evolutionary change. Comparing how genes are regulated in different species is therefore becoming an important part of evo-devo studies. A key tool for investigating the regulation of genes is represented by bacterial artificial chromosomes (BAC)-reporter constructs. BACs are large insert libraries, often >100 kb, which thus capture the geno...

2014
Adam Balic Carla Garcia-Morales Lonneke Vervelde Hazel Gilhooley Adrian Sherman Valerie Garceau Maria W. Gutowska David W. Burt Pete Kaiser David A. Hume Helen M. Sang

We have generated the first transgenic chickens in which reporter genes are expressed in a specific immune cell lineage, based upon control elements of the colony stimulating factor 1 receptor (CSF1R) locus. The Fms intronic regulatory element (FIRE) within CSF1R is shown to be highly conserved in amniotes and absolutely required for myeloid-restricted expression of fluorescent reporter genes. ...

Journal: :BioTechniques 2001
A C Jung M C Criqui S Rutschmann J A Hoffmann D Ferrandon

beta-galactosidase and green fluorescent protein (GFP) are among the most commonly used reporter genes to monitor gene expression in various organisms including Drosophila melanogaster. Their expression is usually detected in a qualitative way by direct microscopic observations of cells, tissues, or whole animals. To measure in vivo the inducibility of two antimicrobial peptide genes expressed ...

Journal: :Methods in molecular biology 2009
Jingjing L Kipp Kelly E Mayo

Use of reporter genes provides a convenient way to study the activity and regulation of promoters and examine the rate and control of gene transcription. Many reporter genes and transfection methods can be efficiently used for this purpose. To investigate gene regulation and signaling pathway interactions during ovarian follicle development, we have examined promoter activities of several key f...

Journal: :Toxicology and applied pharmacology 2006
Jonathan M Shipley David J Waxman

Aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that stimulates transcription directed by xenobiotic response elements upstream of target genes. Recently, AhR ligands were reported to induce formation of an AhR-estrogen receptor (ER) complex, which can bind to estrogen response elements (EREs) and stimulate transcription of ER target genes. Presently, we investigate t...

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