BACKGROUND Acute kidney injury is a common clinical comorbidity and early diagnosis is crucial for improving prognosis, but there is still no ideal biomarker for early diagnosis. MATERIAL AND METHODS miRNA microarray was used for detecting miRNA in kidney subjected to renal ischemia-reperfusion injury 12 h after reperfusion. Real-time PCR was performed to validate the results of microarray. m...

global cerebral ischemia (gci) and reperfusion induced apoptosis that lead to cell injury and death. the bax and bcl-2 are pro-apoptotic and anti-apoptotic genes, respectively. these genes belong to the b-cell lymphoma-2 (bcl-2) family.in this study; we assessed the effect of pentoxifylline drug on bax/bcl2 gene dosage expression changes following   ischemic reperfusion injury in kidney. in thi...

Renal ischemia-reperfusion injury is a major cause of acute renal failure, causing renal cell death, a permanent decrease of renal blood flow, organ dysfunction and chronic kidney disease. Platelet-rich plasma (PRP) is an autologous product rich in growth factors, and therefore able to promote tissue regeneration and angiogenesis. This product has proven its efficacy in multiple studies, but ha...

Fas-mediated apoptosis has been suggested to contribute to tubular cell death after renal ischemia-reperfusion injury. Here we investigate whether small interfering RNA (siRNA) duplexes targeting Fas protect mice from acute renal failure after clamping of the renal artery. Renal ischemia-reperfusion injury was induced by clamping the renal vein and artery for 15 or 35 min. Mice were treated bef...

Background & Aims: Liver ischemia-reperfusion (IR) is one of the common consequences of liver surgery, especially during liver transplantation which results in organ dysfunction. Acute hepatic injury causes systematic inflammatory responses which may finally lead to functional disturbances in remote organs such as heart, lungs and kidneys. In this study, the effects of a potent inhibitor of inf...

Long-term kidney graft survival is affected by different variables including donor condition, ischemia-reperfusion injury, and graft rejection during the transplantation process. The complement system is an important mediator of renal ischemia-reperfusion injury and in rejecting allografts. However, donor complement C3 seems to be crucial in renal transplantation-related injury as renal injury ...

Ischemia/reperfusion injury is the leading cause of acute tubular necrosis. Nitric oxide has a protective role against ischemia/reperfusion injury; however, the role of asymmetric dimethylarginine (ADMA), an endogenous inhibitor of nitric oxide synthase, in ischemia/reperfusion injury remains unclear. ADMA is produced by protein arginine methyltransferase (PRMT) and is mainly degraded by dimeth...

Postconditioning (PostC), a series of brief ischemia/reperfusion (I/R) cycles at reperfusion onset, is a recently described approach to attenuate I/R injury in the heart and brain. Here, we examined its effect on acute kidney injury (AKI) induced by ischemia/reperfusion (I/R) injury in a mouse model. C57/black mice were subjected to right nephrectomy and 26-min left renal artery occlusion, and ...

Purpose: To investigate the effect and possible mechanism of combination of apigenin and ischemic post conditioning on renal ischemia-reperfusion injury in rats. Materials and methods: Fifty rats were randomly separated into 5 groups: (1) sham-operation groups: a midline laparotomy was performed only; (2) I/R group: rats were underwent 45 min of renal ischemia; (3) apigenin group: rats was subj...

CD4+ T cells contribute to the pathogenesis of ischemia-reperfusion injury, which is the primary cause of delayed graft failure after kidney transplantation. The TIM-1:TIM-4 pathway participates in the activation/differentiation of CD4+ T cells, suggesting that it may modulate ischemia-reperfusion injury. Here, we studied the role of TIM-1 in a murine uninephrectomized renal ischemia-reperfusio...