Basidiomycetes are able to biodegrade waste and xenobiotic molecules through the production of extracellular enzymes. For example, white-rot fungi produce lignin-degrading enzymes which are capable of efficiently decolorizing dye solutions. Many mushrooms also produce lectins, a group of proteins which bind specifically to the carbohydrates in glycoconjugates. Several fungal lectins target thei...

This research project aimed to provide an environmentally friendly method for the decolorization and biosorption of synthetic dye by utilizing fungi as biosorbents. The study was carried out first growing in solid medium then using biosorbent absorb aqueous solution. In stage, screening experiments were among 5 different types fungi, Pleurotus ostreatus determined have highest growth rate. recu...

We describe the pre-staining of proteins in normal human serum with Remazol Brilliant Blue before separation by disc electrophoresis. Critical to reproducibility are: dye concentration of 0.16 mol/L in a Tris-glycine buffer (pH 8.3), use of equal volumes of serum and dye solution, a tagging period of 2 h at room temperature, and electrophoresis of 0.1 mL of the mixture at 2.5 mA/gel for about 2...

The proteolytic activity of trypsin releases the dye Remazol Brilliant Blue from its high molecular weight substrate, the skin powder (Hide Powder Azure, Sigma), with an increase in absorbance at 595 nm. Active alpha(2)- macroglobulin (80 mug/ml) totally inhibits the proteolytic activity of trypsin (14 mug/ml) by trapping this protease. But after a 20 min incubation of alpha(2)-macroglobulin at...

We have reported (Clin Chem 29: 42-44, 1983) that prestaining with Remazol Brilliant Blue permits direct visualization of serum components on disc electrophoresis, and apparently purifies the proteins well. Here we have cut out the bands corresponding to the prestained albumin and transferrin after disc electrophoresis of normal human serum proteins, eluted some individual proteins into saline,...

sawdust which is the main waste from wood industry has been used as a raw material forremoval of brilliant cresyl blue toxic dye. the effects of various parameters such as ph,electrolyte concentration, adsorbent dose, contact time and agitation rate were studied for theremoval of the dye in a concentration of 50 mg l-1. the optimum condition for the studiedparameters was applied for various con...