Introduction: Thrombosis and the blockage of blood vessels with clots, can lead to acute myocardial infarction and some times even death. Aside from surgical interventions to remove the blockage, the only available treatment is the administration of thrombolytic agents to dissolve the blood clot. Streptokinase (SK) is the most commonly used fibrinolytic drug for this purpose. However, SK...

  Background and Objectives: Some of the human papillomaviruses (HPVs) can infect genital tracts and are sometimes associated with anogenital tract cancers. HPVs induced cervical cancers through the expression of E6 and E7 genes by inactivating the tumor suppressor proteins. In this study, E6 and E7 genes were chosen in order to construct an expression vector which is able to express ...

Infectious bursal disease virus (IBDV) is the causative agent of Gumboro disease, an infectious disease of global economic importance in poultry. The expression of heterologous proteins in P.pastoris is fast, simple and inexpensive. In this study, VP2 encoding gene of classical D78 IBDV was amplified using reverse transcription (RT) polymerase chain reaction (PCR) and cloned into pPICZαA vector...

In this study, our previously reported novel synthetic gene encoding 166 residues of interferon-? was used for an efficient expression of IFN-?. The synthetic gene was cloned into pET21a expression vector and transferred into E. coli. Recombinant protein was over-expressed in the E. coli. Identity of the recombinant protein was confirmed by western blot analysis. The recombinant protein was bio...

Background: Subunit vaccines are appropriate vaccine candidates for the prevention of some infections. In this study, three immunogenic proteins of Mycobacterium tuberculosis, including HspX, Ppe44, and EsxV as a new construction, were expressed alone and as a fusion protein to develop a new vaccine candidate against tuberculosis infection. Methods: To make the fusion protein, the three genes ...

Bean bruchids are among the most devastating insect pests of common bean that can inflict huge losses in storage. To identify potential resistance to these pests, screening was performed at Sokoine University Agriculture. Two resistant landraces were identified, viz Kalubungula and KK25. Recombinant inbred (RI) KSy, KSw ML populations created from crosses between Soya × Kalubungula, Sowor...

expression of htlv-i p19 protein in an escherichia coli expression system always leads to the formation of inclusion body. solubilisation and refolding of the inclusion bodies is complex, time consuming and difficult during large-scale preparation. this study aimed to express and purify a soluble form of recombinant htlv-i p19 protein in an e. coli expression system. the synthetic dna encoding ...

purpose: in order to express human granulocyte-macrophage colony stimulating factor (hgm-csf) under heat shock. materials and methods: two expression plasmids were constructed based on pbc(sk) plasmid. the expression cassettes in the two plasmids are equipped with a 75 base pair fragment, derived from the pl promoter of the bacteriophage lambda (λ). the plasmids also contain a temperature muta...