objective(s):  infection by high-risk papillomavirus is regarded as the major risk factor in the development of cervical cancer. recombinant dna technology allows expression of the l1 major capsid protein of hpv in different expression systems, which has intrinsic capacity to self-assemble into viral-like particles (vlp). vlps are non-infectious, highly immunogenic and can elicit neutralizing a...

Creatinine amidohydrolase(EC 3.5.2.10) catalyzes the reversible conversion of creatinine to creatine. Creatininase in combination with other enzymes is used for detection of creatinine in serum and urine which is of significant value for detection of renal, muscular and thyroid functions. The aim of this study was to produce recombinant creatininase enzyme in E.coli expression system to use it ...

Background: Staphylococcal protein A (SPA) is a cell wall component of Staphylococcus aureus that binds to different IgG subclasses of human and several animal species. This bacterial protein can be used as an antibody detector in various immunological assays or as an isolation reagent for the purification of antibody molecules via immuno-chromatography procedures.Objectives: Molecular cl...

Background: Recombinant vaccine technology is one of the most developed means in controlling infectious diseases. However, an effective vaccine against Shigella is still missing. Objective: To evaluate recombinant IpaC protein of Shigella as a vaccine candidate. Methods: In this study we cloned IpaC gene into an expression vector in prokaryotic system. The protein expression was evaluated by SD...

chaperones are produced by prokaryotic, yeast and higher eukaryotic cells for various purposes. over-expression of each chaperone or sets of them affect the production level of a recombinant protein in the cell. on the basis of this hypothesis, five different plasmids with 5 different combinations of 6 chaperones molecule, transformed into escherichia coli along with human basic fibroblast grow...

after using 3 different generations of antibodies including human and non-human hyperimmune sera, monoclonal antibodies and chimeric antibodies, more recently a newer approach has been developed in which the antibody genes are cloned directly from a patient peripheral b-lymphocytes and expressed in a host like e. coli. in this study the candida albicans serotype a (nctc 3153) mannan was purifie...

background: detection of hepatitis c virus specific antibodies is the initial step in chronic hcv diagnosis.  hcv ns4b is among the most immunogenic hcv antigens and has been widely used in commercial enzyme immunoassays (eia). additionally, ns4b, a key protein in the virus replication, can be an alternative target for antiviral therapy.objectives: development of a new method for high-level exp...

bioconversion of cellulosic material into bioethanol needs cellulase complex enzymesthat contain endoglucanase, exoglucanase and beta glucosidase. one of the most important organisms that produce cellulases is the filamentous fungi, trichoderma reesei which able to secrete large amounts of different cellulases. these enzymes are probably the most widely used cellulases industrially, however, th...