objective: leishmaniasis is caused by parasitic protozoa of the genus leishmania which in the infected host is obligate intracellular parasite. lack gene is conserved among related leishmania species. lack is the immuno-dominant antigen of l.major which is considered as the most promising molecule for a recombinant or dna vaccine against leishmaniasis. materials and methods: in this study the ...

background pe5 is a member of the pe protein family whose precise function is yet to be understood. there are about 100 members of the pe family proteins in mycobacterium tuberculosis. these glycine- and alanine-rich proteins consist of proline-glutamate motifs at their n-termini and may be implicated in pathogenesis of the bacilli. objectives we aimed to clone and over-express the rv0285 codin...

In this placebo-controlled phase II randomized clinical trial, we evaluated the safety and immunogenicity of a therapeutic prime-boost vaccine strategy using recombinant DNA (GTU-MultiHIV B clade) followed by boost vaccination with lipopeptide (HIV-LIPO-5) in HIV-infected patients on combined antiretroviral therapy. We show here that is well tolerated, consistently previous studies HIV-1-infect...

Once a recombinant DNA is inserted into bacteria, these bacteria will make protein based on this recombinant DNA. This protein is known as “Recombinant protein”. The demand of recombinant proteins has increased as more applications in several fields become a commercial reality [1]. For instance, today, more than 75 recombinant proteins are utilized as pharmaceuticals, and more than 360 new medi...

Once a recombinant DNA is inserted into bacteria, these bacteria will make protein based on this recombinant DNA. This protein is known as “Recombinant protein”. The demand of recombinant proteins has increased as more applications in several fields become a commercial reality [1]. For instance, today, more than 75 recombinant proteins are utilized as pharmaceuticals, and more than 360 new medi...

Once a recombinant DNA is inserted into bacteria, these bacteria will make protein based on this recombinant DNA. This protein is known as “Recombinant protein”. The demand of recombinant proteins has increased as more applications in several fields become a commercial reality [1]. For instance, today, more than 75 recombinant proteins are utilized as pharmaceuticals, and more than 360 new medi...

background and objectives: clostridium perfringens, a gram-positive obligate anaerobic bacterium, is able to form resistant spores which are widely distributed in the environment. c. perfringens is subdivided into five types a to e based on its four major alpha, beta, epsilon and iota toxins. the aim of the present study was cloning and expression of c. perfringens type d vaccine strain epsilon...

Background: Today, several vaccines have been developed to prevent infectious diseases. The older first-generation vaccines may have many problems. In this regard, genetic engineering plays an important role using tools such as shuttle vectors to develop recombinant DNA vaccines that usually include plasmid constructed so that can propagate in two different host species. The present study revie...