This study aimed to determine the causes of bitterness problems in white cheese produced by ultrafiltration (UF). To investigate association between with enzyme use or starter culture, was using UF-pasteurized and UF-raw milk two different coagulating enzymes cultures proteolytic activities. Sensory analysis, protein, water-soluble nitrogen, 12% trichloroacetic acid-soluble 5% phosphotungstic n...

DNA complementary to calf stomach mRNA has been synthesised and inserted into the Pst1 site of pAT153 by G-C tailing. Clones containing sequences coding for prochymosin were recognised by colony hybridisation with cDNA extended from a chemically synthesised oligodeoxynucleotide primer, the sequence of which was predicted from the published amino acid sequence of calf prochymosin. Two clones wer...

The purpose of this study was to investigate the circadian changes in the abomasal secretions (quantities of juice, enzymes and electrolytes) of the preruminant calf. The gastric juice was collected every hour from five calves fitted with an innervated abomasal pouch and given milk twice daily, with a 7-h interval between the morning and the evening meals. The amounts of gastric secretions prod...

results the results showed that sensitivity and specificity were respectively 84.12% and 92% for‎ recombinant protein srs3 and 92% and 96% for rsag1. conclusions recombinant proteins srs3 and rsag1 are promising antigens in diagnostic assays for detection of specific antibodies against t. gondii. background infections by the protozoan parasite toxoplasma gondii (t. gondii) are widely prevalent ...

prochymosin is one of the most important aspartic proteinases used as a milk-clotting enzyme in cheese production. in the present investigation we report sequence of cdna encoding goat ( capra hircus ) preprochymosin and compare its nucleotide and deduced amino acid sequences with sequences of other ruminants preprochymosin. as bovine prochymosin, the caprine prochymosin cdna encodes 365 amino ...

Nutritional properties of inulin-enriched tofu obtained after hydrothermal cooking soymilk, using chymosin-pepsin rennet and inulin as a functional ingredient, were assessed. This procedure significantly differs from the traditional one. The residual activity (rTIA) trypsin inhibitors (TIs) lectins, content proteins, carbohydrates, fats their energy values (EV) suitable for human nutrition. Inu...

Aqueous two-phase systems (ATPS) that are formed by mixing a polymer (usually polyethylene glycol, PEG) and a salt (e.g. phosphate, sulphate or citrate) or two polymers, and water can be effectively used for the separation and purification of proteins. The partitioning between both phases is dependent on the surface properties of the proteins and on the composition of the two phase system as ha...