Yersinia enterocolitica is an important zoonotic pathogen, which seriously endangers food-safety risk. In this study, the recombinant outer membrane protein OmpF and its antibody were prepared coupled with immunomagnetic beads (IMBs) to capture Y. in food samples, combining quantitative PCR detection primers of virulence factor gene foxA for contamination. The results showed that efficiency app...

  Objective(s):  Infection by high-risk papillomavirus is regarded as the major risk factor in the development of cervical cancer. Recombinant DNA technology allows expression of the L1 major capsid protein of HPV in different expression systems, which has intrinsic capacity to self-assemble into viral-like particles (VLP). VLPS are non-infectious, highly immunogenic and can elicit neutralizing...

In this issue of Cell Chemical Biology, Hentrich et al., 2021Hentrich C. Kellmann S.J. Putyrski M. Cavada Hanuschka H. Knappik A. Ylera F. Periplasmic expression SpyTagged antibody fragments enables rapid modular assembly.Cell Chem. Biol. 2021; 28 (this issue): 813-824.e6Abstract Full Text PDF PubMed Scopus (5) Google Scholar describe the application SpyCatcher technology to discovery and valid...

Background: Bovine follicle stimulating hormone (bFSH) belongs to the family of glycoprotein hormones that are released from the pituitary gland or the placenta. This hormone is a heterodimer consisting of common α- and specific β-subunit. Bovine FSH is used for reproductive technology such as superovulation in farm animals and polycystic ovary syndrome treatment. Also this hormone is produced ...

rotavirus nonstructural glycoprotein nsp4 can induce diarrhea in newborn mice. it has been suggested that nsp4 may be a key determinant for rotavirus pathogenesis and a target for vaccine development. in order to study the biological and morphological role of nsp4 a large amount of the purified protein and antibody against it are required. simian rotavirus sa11 was propagated in bscl cell, puri...

Background: The antibody response to hepatitis B surface antigen (HBsAg) controls hepatitis B virus infection. The "a" determinant of HBsAg is the most important target for protective antibody response, diagnosis and immunoprophylaxis. Mutations in this area may induce immune escape mutants and affect the performance of HBsAg assays. Objectives: To construct clinically relevant recombinant muta...

BACKGROUND Recombinant antibody fragments are promising alternatives to full-length immunoglobulins and offer important advantages compared with conventional monoclonal antibodies: extreme specificity, higher affinity, superior stability and solubility, reduced immunogenicity as well as easy and inexpensive large-scale production. OBJECTIVE In this article we will review and discuss recombina...

background: rabies virus (rabv) is a deadly neurotropic virus that causes the disease of rabies in humans and animals. l protein is one of the large structural protein of rabies virus, which displays multiple enzymatic activities, and is required for viral transcription and replication.objectives: a truncated l protein of rabies virus is being cloned, expressed and purified to produce relevant ...