bordetella pertussis infects the respiratory tract of the human host and causes whooping cough in children. the nature of immunity against bordetella pertussis infection and disease is poorly understood. the aim of this study was to investigate cell mediated immunity in mice immunized with outer membrane component of cell wall, of b. pertussis. a group of mice were immunized with outer membrane...

a gradient reversed-phase high performance liquid chromatography (hplc) method was developed for the assay of cetrorelix acetate, a synthetic decapeptide with gonadotropin-releasing hormone (gnrh) antagonistic activity used in infertility treatment. the hplc method, which is used to determine cetrorelix in bulk and pharmaceutical dosage forms, was validated per ich guidelines. the chromatograph...

canine ehrlichiosis is a zoonotic rickettsial disease transmitted by ticks. in the present study, 198 companion dogs of different ages were examined for serum antibody detection against ehrlichia canis by means of immunochromatography assay. the dogs were selected among referred cases to veterinary hospital of shahid chamran university of ahvaz, southwestern iran from november 2008 to march 201...

cancer is a major global problem and is the second leading cause of mortality in the developed countries.resistance to current chemotherapeutics and high incidence of adverse effects are the two principal reasons for developing new anticancer agents. phenylacetamide derivatives can act as potential anticancer agents. synthesis and screening of 2-(4-fluorophenyl)-n-phenylacetamide derivatives in...

two capillary zone electrophoresis (cze) methods were developed in this study, one using an acidic buffer (sodium citrate ph = 2.5) and the other using a basic buffer (sodium tetraborate ph = 9.8). the two methods were compared on the basis of repeatability and reproducibility of results and the cze method developed with the basic buffer was then selected for further studies. the method was ful...

conclusions: it was concluded that the elisa is a suitable method for large scale screening of antibodies against aspergilloma. the easy techniques have shortened the required time of tests (approximately 1.30 hours for our elisa, as opposed to 2.30 hours with commercial elisa kit). this may be a substantial benefit for large numbers of samples that should be tested. materials and methods: an i...