Background: Acinetobacter baumannii is an important pathogen in health care and is responsible for severe nosocomial and community-acquired pneumonia. To design novel therapeutic agents, a mouse model for A. baumannii pneumonia is essential. Methods: We described a mouse model of A. baumannii using clinical and 19606R standard strains for developing a quantitative real-time PCR (qRT-PCR) for ra...

Purpose: To determine the inhibitory effect of curcumin on expression brain-derived neurotrophic factor (BDNF) in hippocampus mice with sleep deprivation.
 Methods: Expressions BDNF and miRNA-206 were evaluated using quantitative real time-polymerase chain reaction (qRT-PCR) a sleep-deprived mouse model. Interaction was assessed by dual luciferase qPCR) assays. The also determined qRT-PCR....

Mulberry (Morus alba L.) is an important economic tree species in many countries. Quantitative real time PCR (qRT-PCR) has become a widely used method for gene expression studies in plants. A suitable reference gene is essential to ensure accurate and reliable results for qRT-PCR analyses. However, no reports describing the selection of reference genes have been published for mulberry. In this ...

Obtaining quantitative data concerning gene expression is important for understanding milk synthesis in mammary glands. Quantitative real-time PCR (qRT-PCR) is an efficient tool to calculate gene expression; however, it is necessary to find valid reference genes for normalization of qRT-PCR data. We applied the geNorm software to eight commonly used reference genes to identify the most stable a...

Zur Detektion SARS-CoV-2-Infizierter („severe acute respiratory syndrome coronavirus 2“) ist die quantitative Real-Time-PCR (qRT-PCR) Goldstandard. Antigenschnelltests haben gegenüber der PCR-Diagnostik den Vorteil, dass sie schnell Ergebnisse liefern und vor Ort durchführbar sind. Nachteil Schnelltests ihre schlechtere Sensitivität Spezifität. Mittlerweile sind mehrere hundert auf BfArm-Liste ...

BACKGROUND Enteropathogenic Escherichia coli (EPEC) strains are pediatric pathogens commonly isolated from both healthy and sick children with diarrhea in areas of endemicity. The aim of this study was to compare the bacterial load of EPEC isolated from stool samples from children with and without diarrhea to determine whether bacterial load might be a useful tool for further study of this phen...