Streptococcal pullulanases have been recently proposed as key components of the metabolic machinery involved in bacterial adaptation to host niches. By sequence analysis of the Group B Streptococcus (GBS) genome we found a novel putative surface exposed protein with pullulanase activity. We named such a protein SAP. The sap gene is highly conserved among GBS strains and homologous genes, such a...

There is an increasing interest in applying the technology of electrospinning for making ultrafine fibers from biopolymers for food-grade applications, and using pullulan (PUL) as a carrier to improve the electrospinnability of proteins and other naturally occurring polyelectrolytes. In this study, PUL solutions containing NaCl or Na3C6H5O7 at different concentrations were electrospun. The incl...

identification and use of more efficient enzymes in the food and pharmaceutical industries is the focus of many researchers. the aim of this study was to search for a new bacterial strain capable of producing high levels of pullulanase applicable to biotechnology, the starch bioprocessing and food industries. a new pullulan hydrolyzing bacillus strain was isolated and designated sdk2. morpholog...

Pullulan, a water soluble, bioadhesive and biocompatible biopolymer is used as an efficient matrix for enalapril maleate film preparation. The obtained film contains as much as 67% of enalapril maleate, relative to the initial amount used for experiments. The drug content was evaluated by using 1 H-NMR and UV-vis spectroscopy. The pullulanenalaprilmaleate blends exhibit good physicochemical pro...

A thermostable pullulanase (alpha-dextrin 6-glucanohydrolase [EC 3.2.1.41]) from a newly isolated Bacillus stearothermophilus strain (TRS128) was purified and characterized. The enzyme hydrolyzed (1-->6)-alpha-d-glucosidic linkages of pullulan to produce maltotriose, and the optimum temperature was 65 degrees C. About 90% of the enzyme activity was retained after treatment at 65 degrees C for 6...