The unprecedented scale and content of genomic and proteomic information now emerging from global sequencing and analysis efforts offer new opportunities in biological research. Technological and computational developments have enabled first phase analytical platforms for genomic and proteomic studies. However, the ultimate goal will be to integrate data from diverse analytical platforms, so th...

S-Nitrosation plays an important role in regulation of protein function and signal transduction. Discovering S-nitrosated targets is a prerequisite for further functional study. However, current proteomic methods used to quantify S-nitrosation are limited in their applicability to certain types of samples, or by the need for special reagents and complex procedures to obtain the results. Here we...

Down's syndrome (DS) is the most common genetic cause of developmental delay with an incidence of 1 in 800 live births, and is the predominant reason why women choose to undergo invasive prenatal diagnosis. However, as invasive tests are associated with around a 1% risk of miscarriage new non-invasive tests have been long sought after. Recently, the most promising approach for non-invasive pren...

PURPOSE Our study was undertaken to determine the utility of plasma proteomic profiling using surface-enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry for the detection of head and neck squamous cell carcinomas (HNSCCs). EXPERIMENTAL DESIGN Pretreatment plasma samples from HNSCC patients or controls without known neoplastic disease were analyzed on the Protein...

Early detection and effective treatment of severe COVID-19 patients remain two major challenges during the current pandemic. Analysis molecular changes in blood samples is one promising approaches to this problem. From thousands proteomic, metabolomic, lipidomic, transcriptomic biomarkers selected other research, we identify several pairs that after additional nonlinear spline transformation ar...

BACKGROUND The ability to predict mode, as well as risk, of death in left ventricular systolic dysfunction (LVSD) is important, as the clinical and cost-effectiveness of implantable cardioverter defibrillators (ICD) therapy depends on its use in appropriately selected patient populations. The value of a proteomic approach in identifying prognostic biomarkers in LVSD is unknown. The aims of this...

High-throughput screening for interactions of peptides with a variety of antibody targets could greatly facilitate proteomic analysis for epitope mapping, enzyme profiling, drug discovery and biomarker identification. Peptide microarrays are suited for such undertaking because of their high-throughput capability. However, existing peptide microarrays lack the sensitivity needed for detecting lo...