نتایج جستجو برای: proteolytic extract

تعداد نتایج: 198603  

Journal: :Toxicon : official journal of the International Society on Toxinology 2010
E A B Undheim D N Georgieva H H Thoen J A Norman J Mork C Betzel B G Fry

The venom of Antarctic octopus remains completely unstudied. Here, a preliminary investigation was conducted into the properties of posterior salivary gland (PSG) extracts from four Antarctica eledonine (Incirrata; Octopodidae) species (Adelieledone polymorpha, Megaleledone setebos, Pareledone aequipapillae, and Pareledone turqueti) collected from the coast off George V's Land, Antarctica. Spec...

Journal: :Journal of experimental zoology. Part A, Ecological genetics and physiology 2012
Catherine Jarrett Kemp Dietmar Kültz

An attempted proteomic study of Daphnia pulex response to sodium chloride exposure revealed an unexpected result: very strong proteome degradation previously observed in D. pulex is halted when animals have been exposed to elevated salinity prior to protein extraction. Further characterization of conditions controlling proteome degradation shows that the proteolytic factor(s) is/are present in ...

Journal: :BioTechniques 2000
C Mariac P Trouslot C Poteaux G Bezançon J F Renno

The technique described here is a fast and simple method of extracting chloroplast DNA (cpDNA). It overcomes the need for differential centrifugation using density gradients. The leaves do not have to be kept in the dark and lyophilized before extraction, but lyophilization is still possible. The chloroplasts are specifically lysed in a cell extract of leaves, using a non-ionic detergent. After...

Journal: : 2023

In recent years, plant growth promoting bacteria (PGPB) have been used as biofertilizers that increase agricultural productivity and resistance to changing environmental conditions such drought, salinity heat stress. One of the proposed new approaches productivity, especially under stress conditions, is using algae extracts, which usually consist naturally occurring bioactive compounds. Paeniba...

Journal: :journal of sciences, islamic republic of iran 2013
m. banijamali s. hosseinkhani j. fuladi

pyruvate kinase is a key enzyme in glycolytic pathway that catalyzes the transphosphorylation between phosphoenolpyruvate and adp to yield atp and pyruvate. geobacillus stearothermophillus has a stable pyruvate kinase with determined crystal structure that composed of four separate domains. given that limited proteolysis experiments can be successfully used to probe conformational features of p...

Journal: :Iranica journal of energy and environment 2021

Alkaline proteases are the most important groups of commercial enzymes, which have been broadly used in industrial processes. In this study, Bacillus sp. PTCC 1538 was selected as a biological agent to produce alkaline protease. Enzyme production under submerge fermentation using waste effluent investigated. Since costs raw material plays an role cost enzyme production, corn steep liquor (CSL) ...

Journal: :The Biochemical journal 1990
Y Mikuni-Takagaki M J Glimcher

In order to understand the mechanism of the post-translational processing of bone phosphoproteins in embryonic bone, periosteal bone strips isolated from 12-day-embryonic-chick tibiae were cultured and the bone proteins labelled with Na2H32PO4. Of the total radiolabelled proteins recovered from the medium and bone extracts in the absence of SDS ('medium', 'EDTA extract' and 'EDTA/guanidinium ch...

2016

Aspergillus flavus produces a lipase (EC 3.1.1.3) which is partly bound to the mycelium during growth. The production of the mycelium-bound lipase is concomitant with growth, and declines when growth ceases. Maximum productivity of the enzyme is obtained when the culture is incubated at 30°C, an initial culture pH of 6·5 and with 2% (w/v) each of corn oil and yeast extract as carbon and organic...

Journal: Current Medical Mycology 2016

Background and Purpose: Globally, dermatophytes are the most common filamentous group of fungi causing cutaneous mycoses. Dermatophytes were shown to secrete a multitude of enzymes that play a role in their pathogenesis. There is limited data on co-hemolytic (CAMP-like) effect of different bacterial species on dermatophyte species. In this study, we sought to the evaluate exoenzyme activity an...

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