sepsis is a significant health problem with an estimated 750,000 new cases in the usa annually. it is also the third leading cause of death in developed countries, equaling the number of fatalities from acute myocardial infarction. the high sepsis-related mortalities mean there is an urgent need to improve the diagnosis and management of sepsis patients. the aim of this study was the evaluation...

in the present study, we investigated total cell protein patterns of ten isolates of epidermophyton floccosum var.pigmented by sds-page on 10% polyacrylamide resolving gels. densitometric analysis of the gels allowed to detect more than 31 clearly detectable mycelial protein bands with molecular weights in the range of 12 to 98 kd proteins of 12.5, 13.5, 14.4, 16, 18.4, 19.7, 20.1, 23.5, 26, 27...

castor bean, peanut, soy bean and safflower protein isolates were prepared. the amino acid content of each of the protein isolates was analysed and the essential amino acid contents were compared with the fao human requirements. the results indicated that castor bean has the highest oil and the protein content of defatted meal. safflower 3148 (marand, iran) has the highest amount of essential a...

abstract         background and objective: hirudin is an anticoagulant polypeptide secreted from the salivary glands of leeches. recombinant hirudin is a strong anticoagulant agent in arterial and venous thrombosis. the aim of this study was to evaluate the effect of inserting protein a signal peptide sequence of pezz18 plasmid on expression and secretion of the recombinant hirudin in e.coli.  ...

the aim of this study was to compare three routine precipitation methods, including: acetone, tca/acetone wash and tca/acetone. 30 plasma samples were precipitated using above mentioned three methods. pellets were dissolved in rehydration solution and protein quantification was done for this solution. according to statistical analysis using spss ver 16.0 software and one-way anova test, the pro...

background rapid detection of resistant strains of helicobacter pylori in human clinical samples is of major importance in clinical settings. inability of conventional clinical laboratory techniques in detection of these strains usually leads to failure of prescribed therapeutic regimens. objectives the aim of this study was designing a simple and rapid allele-specific pcr (as-pcr)-based method...

adenylyl cyclase is a membrane-bound enzyme that catalyzes the conversion of atp to camp. the inhibition of adenylyl cyclase was carried out by measuring the ability of the macrophage chemotactic protein-1 to inhibit the torskolin-induced enzyme activity. adenylyl cyclase activity in the presenc of macrophage chemotactic protein-1 was decreased compared to that in controls [2.11+/-0.15 (mean +/...

objective(s):brucellosis, especially caused by brucella melitensis, remains one of the most common zoonotic diseases worldwide with more than 500,000 human cases reported annually. the commonly used live attenuated vaccine in ovine brucellosis prophylaxis is b. melitensis rev1. but due to different problems caused by the administration of this vaccine, a protective subunit vaccine against b. me...

background and objective: chlorella vulgaris is a multi-cellular edible algal species with abundant proteins. extraction of high value protein fractions for pharmaceutical and nutritional applications can significantly increase the commercial value of microalga biomasses. there is no known report on the anticancer peptides derived from the chlorella vulgaris abundant protein. materials and meth...

conclusions these results demonstrated that the recombinant lysostaphin produced in this study was able to kill nasal s. aureus in rats. it can be recommended for human clinical trial studies. results recombinant lysostaphin at 100 μg/ml concentration showed a significant decrease of the optical density compared to the control samples. the in vivo study demonstrated that a single dose (200 μg/d...